Cobas ampliprep cobas taqman 48 system

A fasting venous blood sample was collected and analyzed for total cholesterol, LDL-cholesterol, HDL-cholesterol, HbA1c, and glucose[35 (link),37 (link)]. CD4+ T-cell count (cells/uL) was measured as a routine analysis by flow cytometry as previously described[35 (link)]. HIV viral load (copies/mL) was measured with a polymerase chain reaction (PCR) quantitative kit (COBAS^®AmpliPrep/COBAS^®TaqMan^® 48 System; Roche, Basel, Switzerland) according to the manufacturer’s instructions. Nadir CD4 was recorded as the lowest CD4+ T-cell count measured during HIV-infection[35 (link)]. As previously described, plasma concentrations of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), were measured by Meso-Scale Discovery MULTI-SPOT plate (MSD, Gaithersburg, MD)[35 (link)], and T-cell subsets (percentage of activated (CD38+HLA-DR+) and senescent (CD28-CD57+) CD8+ T-cells) were determined using a six-colour FACSCanto flow cytometer (Becton Dickinson, Franklin Lakes, NJ)[35 (link)].

Quantitative HBV - DNA real - time PCR tests were completed using a COBAS AmpliPrep / COBAS Taqman 48 system (Roche, Branchburg, NJ, USA). Viral nucleic acids were extracted from 500 µl patient serum with a COBAS AmpliPrep automated extractor according to the manufacturer’s instructions. The measurement interval of the kit for HBV- DNA is 20 - 1.7 x 10⁸ IU/ml.