The symbiotic interaction of wild-type Bradyrhizobium DOA9 and derivatives with A. americana was analyzed as described by Wongdee et al. (2016 (link), 2018) (link). At 20 days post-inoculation (dpi), the numbers of nodules on the roots were determined, and the nitrogenase enzyme activity of the plants was measured using the acetylene reduction assay (ARA). For cytological analysis, fresh nodules were sliced into 30-micron sections using a vibratome (Leica VT1000S Vibrating blade microtome, United States). Nodule sections of plants inoculated with DOA9-PmrpoN strains were stained with X-gluc (Bonaldi et al., 2010 (link)). After staining, the sections were mounted on microscope slides and observed by light microscopy. Nodule section of plants inoculated with rpoN mutants were stained with Live-Dead staining reagent (Invitrogen), after which they were analyzed using confocal microscopy as described by Okazaki et al. (2016) (link).
Live dead staining reagent
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The Live/Dead staining reagent is a fluorescent dye-based solution used to differentiate between live and dead cells in a sample. It provides a rapid and accurate method for assessing cell viability. The reagent contains a combination of fluorescent dyes that selectively stain live and dead cells, enabling easy visualization and quantification under a microscope or flow cytometer.
Automatically generated - may contain errors
3 protocols using live dead staining reagent
1
Symbiotic Interaction Analysis of Bradyrhizobium and A. americana
2
Evaluating Cell Viability by Confocal Microscopy
3
Live/Dead Cell Quantification by Confocal Microscopy
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