Metal solutions, Ni(NO3)2, Pb(NO3)2, Cd(NO3)2 and Cr(NO3)2 (Sigma-Aldrich, USA), were sterilized by filtration through 0.45μm Millipore bacterial filters (Advantec, Tokyo, Japan). Metal-resistant profiles of LAB isolates were conducted on MRS agar plates amended with metals concentrations (up to 600 ppm) and incubated under anaerobic conditions at 30 °C for 1–3 days. The minimum inhibitory concentrations (MICs) were assessed by determining the lowest metal concentrations that completely inhibited LAB growth39 (link). The higher-growth-exhibiting isolates were selected as potential metal-resistant LAB isolates for further studies.
Ni no3 2
Manufactured by Merck Group
Sourced in United States, Germany
Ni(NO3)2, or nickel nitrate, is a chemical compound with the formula Ni(NO3)2. It is a green crystalline solid that is soluble in water. Ni(NO3)2 is commonly used in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Cu no3 2, by Merck Group (10 mentions)
Pb no3 2, by Merck Group (8 mentions)
Cd no3 2, by Merck Group (8 mentions)
Zn no3 2, by Merck Group (6 mentions)
Mg no3 2, by Merck Group (6 mentions)
Co no3 2, by Merck Group (5 mentions)
Hydrochloric acid (hcl), by Merck Group (5 mentions)
Hg no3 2, by Merck Group (5 mentions)
Sodium hydroxide (naoh), by Merck Group (4 mentions)
Mn no3 2, by Merck Group (4 mentions)
Fe no3 3, by Merck Group (4 mentions)
Agno3, by Merck Group (3 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
Ca no3 2, by Merck Group (3 mentions)
N n dimethylformamide (dmf), by Merck Group (2 mentions)
Na2co3, by Merck Group (2 mentions)
Cuso4, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (1 mentions)
Adam17, by R&D Systems (1 mentions)
Ethanol, by Merck Group (1 mentions)
23 protocols using ni no3 2
1
Screening Metal-Resistant Lactic Acid Bacteria
2
Preparation of Metal Nitrate Solutions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AgNO3, Cu(NO3)2, Co(NO3)2, Ni(NO3)2, and Zn(NO3)2 were analytical grade compounds from Sigma Aldrich. We used Acetonitrile for HPLC “Gradient grade” (Sigma Aldrich) and Methanol (J. T. Baker) for HPLC “Gradient Grade”. All solutions in methanol and Acetonitrile (10−3 M each) were prepared daily prior to dilution to 10−4 M for mass spectrometric investigation (ligand/metal salt ratio 1:1). In each case, the freshly prepared solutions were used.
3
Synthesis of Porous NiO Nanoplates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Konjac glucomannan (KGM, Shimizu Chemical Co., Japan), nickel nitrate (Ni(NO3)2, Sigma-Aldrich) and C2H5OH (96 vol%, Quangzi, China) were purchased and used without further purification.
In a typical experiment, 1 g of KGM was dissolved in 50 mL of deionized water by magnetic stirring for 30 min to form a KGM gel. After, the obtained KGM gel was mixed with 50 mL of ethanol for 10 min to form KGM nanofibril deposition. The KGM nanofibrils were taken out and washed with ethanol several times. After that, KGM nanofibrils were immersed in 30 mL of ethanol solution containing 0.03 mol Ni(NO3)2 for 24 h. The Ni2+ absorbed KGM nanofibrils were taken out and washed with ethanol several times. The Ni–KGM composites were dried at 50 °C for 15 h in an oven and then calcined at 600 °C for 6 h with a heating rate of 2 °C min−1 to obtain highly porous NiO nanoplates. The schematic diagram of the production of porous NiO nanoplates is shown inScheme 1 .
In a typical experiment, 1 g of KGM was dissolved in 50 mL of deionized water by magnetic stirring for 30 min to form a KGM gel. After, the obtained KGM gel was mixed with 50 mL of ethanol for 10 min to form KGM nanofibril deposition. The KGM nanofibrils were taken out and washed with ethanol several times. After that, KGM nanofibrils were immersed in 30 mL of ethanol solution containing 0.03 mol Ni(NO3)2 for 24 h. The Ni2+ absorbed KGM nanofibrils were taken out and washed with ethanol several times. The Ni–KGM composites were dried at 50 °C for 15 h in an oven and then calcined at 600 °C for 6 h with a heating rate of 2 °C min−1 to obtain highly porous NiO nanoplates. The schematic diagram of the production of porous NiO nanoplates is shown in
4
Metalloproteinase ADAM17 Enzymatic Assay
5
Biochar Preparation from Corn Stover
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PAN (Mw = 150,000), poly(vinyl alcohol) (PVA, Mw = 13,000~23,000), N,N-dimethylformamide (DMF), ethanol, acetone, and Ni(NO3)2 were purchased from Sigma-Aldrich (St. Louis, MO, USA). The unhydrolyzed solids (UHS), which were derived from alkaline pretreated corn stover, underwent the hydrothermal liquefaction (HTL) process at 300 °C under the reactor pressure of 1600 psi to prepare the biochar samples. During the HTL process, Ni(NO3)2 was utilized as the catalyst and the UHS to deionized water ratio was set at 1:10. After the HTL process, the resulting biochar was rinsed with acetone to extract bio-oils and then treated at 400 °C for 2 h (under inert atmosphere) to remove volatile compounds and residual bio-oils retained after acetone extraction. Thereafter, the biochar powder was dispersed in ethanol and then added into a high-speed blender (i.e., a Waring Laboratory Blender). The biochar particle suspension was mechanically blended for 20 min and then transferred into a glass flask and dried for further use.
6
Peptide D-12 Binding Interaction Assays
7
Ni2+-Catalyzed Peptide Hydrolysis Kinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hydrolysis experiments were performed in a 20 mM HEPES buffer (Sigma-Aldrich), using 0.5 mM peptide and 2 mM Ni(NO3)2 (Sigma-Aldrich). The samples were incubated in pH 8.2, at 50°C and pH 7.4, at 37°C. The aliquots were periodically collected from the samples and acidified by addition 2% (v/v) TFA. Control samples, containing peptide and buffer, but without Ni2+, were gathered at the same time points. For analysis, reaction mixtures were diluted by water 4 to 1 and injected into the HPLC system (Waters), equipped with an analytical C18 column. The eluting solvent A was 0.1% (v/v) TFA in water, and solvent B was 0.1% (v/v) TFA in 90% (v/v) acetonitrile. The chromatograms were obtained at 220 and 280 nm. After separation, the products of hydrolysis were identified using electrospray ionization mass spectrometry (ESI-MS). The relative amounts of these fractions in each chromatogram were calculated by peak integration using data analysis software Origin 8.1 or Origin Pro 2017 (OriginLab Corporation).
8
Nickel-Induced Keratinocyte Differentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Normal human epidermal keratinocytes (NHEKs) (purchased from Lonza) were cultured in monolayers in a dedicated medium (Lonza, KBM-2) at the Ca2+ level of 0.06 mM. To stimulate differentiation and FLG expression, a calcium switch was conducted over a period of 24 h by replacing the culture media with fresh media adjusted to a 1.5 mM final calcium concentration. A Ni(NO3)2 (Sigma) solution was added to achieve various final concentrations (10 μM, 100 μM and 1 mM). Doses were chosen based on MTT test results (Supplementary Figure S1 ). After 24 h of incubation, the cells were fixed, permeabilized and immunostained with anti-FLG antibodies (Anti-FLG goat G20 (Santa Cruz), and secondary anti-goat Alexa-488 and anti-rabbit Alexa-568 (Life Technologies) antibodies were used. Staining was carried out in PBS and nuclei were visualized by Hoechst (NucBlue, Life Technologies). The slides were coversliped with Mowiol 4-88 (Sigma). Data acquisition was carried out on the Zeiss 780 inverted confocal microscope. Images from three separate experiments were analysed; KHG diameter and integrated intensity from the signal were measured using Fiji: ImageJ program (Abramoff, 2007 (link)). For the statistical analysis the Mann–Whitney U test was used.
9
Electrochemical Analysis Using PCTFE Cell
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Electrolytes were either prepared
from NaClO4 (99.99%, trace metal basis, Sigma-Aldrich),
HClO4 (60% by wt. solution, EMSURE, Merck), KOH (86.7%,
analytical reagent grade, Fisher Scientific), NaOH (>98%, Puriss,
Sigma-Aldrich), Ni(NO3)2 (for analysis, Sigma-Aldrich),
Co(NO3)2 (Puratronic, Alfa Aesar), 2-propanol
(Normapur, VWR Chemicals), argon (5.0 purity, Linde), and CO (4.7
purity, Linde).
The dual thin-layer cell was manufactured from
PCTFE and constantly purged with argon to keep ambient air from entering
the vacuum of the mass spectrometer. This allows a steady base line
on masses 32 and 44. A PTFE membrane (PTF002LH0P—Samp, Pall
Inc.) was peeled with a scalpel from the support, and two layers of
it were used to form the vacuum|electrolyte interface. Four layers
of the same PTFE membrane were used as spacers. Electrochemical measurements
were conducted either with an Ivium CompactStat or with a PalmSens
Potentiostat.
During the experiments, the electrolyte is continuously
purged
with argon or CO. Hence, the electrolyte should be saturated with
the respective gas during the electrochemical experiment.
from NaClO4 (99.99%, trace metal basis, Sigma-Aldrich),
HClO4 (60% by wt. solution, EMSURE, Merck), KOH (86.7%,
analytical reagent grade, Fisher Scientific), NaOH (>98%, Puriss,
Sigma-Aldrich), Ni(NO3)2 (for analysis, Sigma-Aldrich),
Co(NO3)2 (Puratronic, Alfa Aesar), 2-propanol
(Normapur, VWR Chemicals), argon (5.0 purity, Linde), and CO (4.7
purity, Linde).
The dual thin-layer cell was manufactured from
PCTFE and constantly purged with argon to keep ambient air from entering
the vacuum of the mass spectrometer. This allows a steady base line
on masses 32 and 44. A PTFE membrane (PTF002LH0P—Samp, Pall
Inc.) was peeled with a scalpel from the support, and two layers of
it were used to form the vacuum|electrolyte interface. Four layers
of the same PTFE membrane were used as spacers. Electrochemical measurements
were conducted either with an Ivium CompactStat or with a PalmSens
Potentiostat.
During the experiments, the electrolyte is continuously
purged
with argon or CO. Hence, the electrolyte should be saturated with
the respective gas during the electrochemical experiment.
10
Impedance Measurements of Salt Solutions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The impedance was measured between 0.125 and 1.00 mM (1 ml, n = 8) for the following compounds on paper discs: BaCl2, CaCl2, Ca(NO3)2, CdCl2, Cd(NO3)2, CdSO4, Cu(NO3)2, CuSO4, GdCl3, K2SO4, LaCl3, Mg(NO3)2, MgCl2, Na2CO3, Na2SO4, NaOH, Ni(NO3)2, and NiCl2 (salts were purchased from Sigma-Aldrich). An extended range (0.01 μM to 0.1 M, n ≥ 15) was measured for the following salts: AgNO3, CuCl2, KCl, KH2PO4, KNO3, NaCl, NaH2PO4, NaNO3, NH4Cl, NH4H2PO4, and NH4NO3. Impedance was measured for at least 2 hours, and the average of the impedance response was recorded. Log10(Z) was plotted against log10(c) and the linear fit was found for each salt.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!