Dm750 led

Manufactured by Leica

The Leica DM750 LED is a compact and efficient light microscope designed for routine laboratory tasks. It features an LED illumination system that provides consistent and long-lasting illumination. The DM750 LED is capable of various magnification levels, allowing users to observe and analyze samples with clarity.

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Lab products found in correlation

Icc50w, by Leica (2 mentions) Colcemid, by Thermo Fisher Scientific (1 mentions) Permount, by Merck Group (1 mentions) Cm1950, by Leica (1 mentions) Fast green fcf, by Merck Group (1 mentions)

Spelling variants of this product

DM750 (288 citations) DM750 LED biological microscope (2 citations)

2 protocols using dm750 led

Bovine iPSC Karyotyping Protocol

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Bovine iPSCs cultured without feeders were treated with 0.1 µg/ml mitotic arrestant, colcemid (Life Technologies) for 16 h. The cells were then rinsed with PBS, and trypsinized to obtain a single cell suspension and pelleted by centrifugation at 100 g for 5 min. The cells were then resuspended in 5 ml hypotonic solution (0.56% KCl), incubated at 37°C for 30 min and fixed with methanol:acetic acid solution (3:1, Carnoy's solution). Drops of the cells in suspension were collected with impact on glass slides that were pretreated with Carnoy's solution (1 min) and washed with ice-cold water. Slides were subsequently air-dried and stained with 5% Giemsa solution for 2 min and rinsed in water, air-dried again and mounted with coverslips using Permount (Sigma-Aldrich). The spreads were imaged using a light microscope (Leica DM750 LED) and high-definition camera (Leica ICC50W), and chromosome numbers in individual spreads were counted.

Pillai V.V., Koganti P.P., Kei T.G., Gurung S., Butler W.R, & Selvaraj V. (2021). Efficient induction and sustenance of pluripotent stem cells from bovine somatic cells. Biology Open, 10(10), bio058756.

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Cryosectioning and Oil Red O Staining

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Unfixed tissues from mice and hamsters were immediately embedded in optimal cutting temperature compound (Sakura Fine-Tek^®) and maintained frozen in −80˚C. Frozen sections (5 μm thickness) were cut using a cryostat (CM1950, Leica), collected on glass slides, and stained for neutral lipid deposits using Oil Red O (Matheson Coleman & Bell). For staining, slides were first fixed with 4% formaldehyde during thaw for 60 minutes at room temperature, and then washed with deionized water to remove mounting compound. Sections were then immersed in Oil Red O dissolved in 60% isopropyl alcohol and incubated for 30 minutes at room temperature. This was followed by washing with deionized water, counterstaining using Fast Green FCF (Millipore Sigma) and mounting with a coverslip using glycerol. Slides were viewed using a light microscope (Leica DM750 LED) and images were acquired using a high definition color camera (Leica ICC50W).

Koganti P.P, & Selvaraj V. (2020). Lack of adrenal TSPO/PBR expression in hamsters reinforces correlation to triglyceride metabolism. The Journal of endocrinology, 247(1), 1-10.

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