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Anti mouse cd4 magnetic beads

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Anti-mouse CD4-magnetic beads are a laboratory tool used for the isolation and separation of mouse CD4-positive cells from a heterogeneous cell population. The beads are coated with antibodies that specifically bind to the CD4 surface marker on mouse T helper cells, allowing for their effective separation and enrichment through magnetic separation techniques.

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Lab products found in correlation

Cd4 cd62l t cell isolation kit, by Miltenyi Biotec (1 mentions) D glucose, by Thermo Fisher Scientific (1 mentions) Ahr inhibitor ch223191, by Merck Group (1 mentions) Tgf β, by Merck Group (1 mentions) Facsaria 2 flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Anti-mouse CD4 Magnetic beads Anti-CD4 Mouse anti

8 protocols using anti mouse cd4 magnetic beads

1

Isolation of Naive CD4+ T Cells

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CD4+ T cells were isolated by using anti-mouse CD4-magnetic beads (BD Biosciences) [24 (link)]. CD4+CD62L+ naïve T cells were isolated by using the CD4+CD62L+ T cell isolation kit from Miltenyi Biotec according to the instructions provided by the manufacturer [25 (link)].
Wu W., Liu H.P., Chen F., Liu H., Cao A.T., Yao S., Sun M., Evans-Marin H.L., Zhao Y., Zhao Q., Duck L.W., Elson C.O., Liu Z, & Cong Y. (2016). Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production. European journal of immunology, 46(5), 1162-1167.
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2

Th17 Cell Polarization from Mouse CD4+ T Cells

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As described previously29 (link), CD4+ T cells were isolated from the spleens of WT and GPR43−/− mice by using anti-mouse CD4-magnetic beads (BD Biosciences). To polarize Th17 cells, CD4+ T cells were cultured with 10 ng/ml TGF-β1, 30 ng/ml IL-6, 10 µg/ml anti-IFN-γ, and 10 µg/ml anti-IL-4 and activated with anti-CD3 and anti-CD28 for 5 d.
Wu W., Sun M., Chen F., Cao A.T., Liu H., Zhao Y., Huang X., Xiao Y., Yao S., Zhao Q., Liu Z, & Cong Y. (2016). Microbiota metabolite short chain fatty acid acetate promotes intestinal IgA response to microbiota which is mediated by GPR43. Mucosal immunology, 10(4), 946-956.
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3

Th17 Cell Differentiation Protocol

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CD4+ T cells were isolated by using anti-mouse CD4-magnetic beads (BD Biosciences) and cultured with irradiated splenic antigen presenting cells (APCs) at 37°C in humid air with 5% CO2 under Th17 polarization conditions with TGF-β (10 ng/mL), IL-6 (30 ng/mL), anti-IFN-γ (10 μg/mL), and anti-IL-4 (5 μg/mL).
Sun M., He C., Chen L., Yang W., Wu W., Chen F., Cao A.T., Yao S., Dann S.M., Dhar T.G., Salter-Cid L., Zhao Q., Liu Z, & Cong Y. (2018). RORγt represses Th17 cell IL-10 production to maintain their pathogenicity in inducing intestinal inflammation. Journal of immunology (Baltimore, Md. : 1950), 202(1), 79-92.
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4

Induction of Murine Th1 Cells

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CD4+ T-cells were isolated from the spleens of mice using anti-mouse CD4-magnetic beads (Cat#: 551539, BD Biosciences). To polarize Th1 cells, CD4+ T-cells were cultured with irradiated splenic antigen-presenting cells (APCs) and CBir1 peptide, or with anti-CD3 and anti-CD28 mAbs, respectively, in the presence of IL-12 (10 ng/ml) for 5 days for two cycles.
Sun M., Wu W., Chen L., Yang W., Huang X., Ma C., Chen F., Xiao Y., Zhao Y., Ma C., Yao S., Carpio V.H., Dann S.M., Zhao Q., Liu Z, & Cong Y. (2018). Microbiota-derived short-chain fatty acids promote Th1 cell IL-10 production to maintain intestinal homeostasis. Nature Communications, 9, 3555.
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5

Th17 Cell Polarization from Mouse CD4+ T Cells

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As described previously29 (link), CD4+ T cells were isolated from the spleens of WT and GPR43−/− mice by using anti-mouse CD4-magnetic beads (BD Biosciences). To polarize Th17 cells, CD4+ T cells were cultured with 10 ng/ml TGF-β1, 30 ng/ml IL-6, 10 µg/ml anti-IFN-γ, and 10 µg/ml anti-IL-4 and activated with anti-CD3 and anti-CD28 for 5 d.
Wu W., Sun M., Chen F., Cao A.T., Liu H., Zhao Y., Huang X., Xiao Y., Yao S., Zhao Q., Liu Z, & Cong Y. (2016). Microbiota metabolite short chain fatty acid acetate promotes intestinal IgA response to microbiota which is mediated by GPR43. Mucosal immunology, 10(4), 946-956.
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6

Th17 Cell Transfer and DSS-Induced Colitis

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CD4+ T cells were isolated from the spleens of CBir1-Tg mice by using anti-mouse CD4-magnetic beads (BD Biosciences) as previously described (23 (link)). To generate Th17 cells, 0.2×106 CD4+ CBirl-Tg T cells were cultured with the same number of irradiated splenocytes in the presence of 10ng/ml TGFβ1, 20ng/ml IL-6, 10μg/ml anti-IFNγ (XMG1.2), and 10μg/ml anti-IL-4 (12B11) for 5 days. The polarized Th17 cells were validated by FACS staining. Then, 2×106 Th17 cells were transferred i.v. into the recipient Rag−/− mice. Mice were monitored by weight weekly and sacrificed either when body weight reached 80% of initial weight or at the end of 6 weeks. As previously described (18 ), 2% DSS (MP Biomedicals) was administrated to Rag−/− mice for seven days, followed by three days of fresh water. Mice were monitored by weight daily and sacrificed either when body weight reached 80% of initial weight or at the end of day 10.
Chen F., Cao A., Yao S., Evans-Marin H.L., Liu H., Wu W., Carlsen E.D., Dann S.M., Soong L., Sun J., Zhao Q, & Cong Y. (2016). mTOR mediates IL-23 induction of neutrophil IL-17 and IL-22 production. Journal of immunology (Baltimore, Md. : 1950), 196(10), 4390-4399.
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7

Modulating Th Cell Differentiation with Glucose and Ahr Inhibitor

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CD4+ T cells were isolated from spleens using anti-mouse CD4-magnetic beads (BD Biosciences). CD4+ T cells were cultured with CBir1 peptides (ThermoFisher Scientific) and irradiated splenic antigen-presenting cells (APCs) or were activated with 5 μg/ml plate-bound anti-mouse-CD3 (Bio X Cell) and 2 μg/ml soluble anti-mouse CD28 (Bio X Cell). Cells were treated with different concentrations of D-glucose (Fisher chemical) in RMPI 1640 medium in the presence or absence of 3 μM Ahr inhibitor CH223191 (Sigma) and cultured under neutral (without exogenous cytokines), Th1 (10 ng/mL IL-12), Th17 (2 ng/mL TGFβ, 50 ng/mL IL6, 10 ug/ml anti-IFNγ mAb, 5 ug/ml anti-IL-4 mAb), or Treg (2 ng/mL TGFβ) polarization conditions. All recombinant antibodies were obtained from Biolegend.
Yu Y., Yang W., Yu T., Zhao X., Zhou Z., Yu Y., Xiong L., Yang H., Bilotta A.J., Yao S., Golovko G., Plasencia A., Quintana F.J., Zhou L., Li Y, & Cong Y. (2022). Glucose promotes regulatory T cell differentiation to maintain intestinal homeostasis. iScience, 25(9), 105004.
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8

Chronic Colitis Induction in Rag1-/- Mice

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The chronic colitis model was established according to our previous report.27 (link) Briefly, splenic CD4+ T cells were separated from Tob1–/– mice and WT littermates using anti-mouse CD4 magnetic beads (BD Biosciences). After surface staining, naïve CD25–CD45RBhighCD4+ T cells of WT littermates and Tob1–/– mice were sorted from splenic CD4+ T cells on a BD FACSAria II Flow Cytometer and then transferred intraperitoneally into 8-week-old Rag1–/– mice (5 × 105 cells/mouse), respectively. These recipient Rag1–/– mice were monitored weekly for clinical characteristics such as weight loss and diarrhea. These recipients were sacrificed after 7 weeks of T cell adoptive transfer. Then, the severity of colitis was assessed according to the changes of clinical characteristics. Colon tissues were collected for hematoxylin and eosin staining, RNA analysis, and LPMC isolation, respectively.
Lin R., Ma C., Fang L., Xu C., Zhang C., Wu X., Wu W., Zhu R., Cong Y, & Liu Z. (2021). TOB1 Blocks Intestinal Mucosal Inflammation Through Inducing ID2-Mediated Suppression of Th1/Th17 Cell Immune Responses in IBD. Cellular and Molecular Gastroenterology and Hepatology, 13(4), 1201-1221.
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