Tetradeuterated methanol (CD 3 OD, 99% at 2 H) was supplied by Aldrich (Steinheim, Germany). Methanol of UHPLC grade, ethyl acetate and 37% hydrochloric acid were purchased from Mallinckrodt Baker (Griesheim, Germany).
Ethyl acetate
Manufactured by Mallinckrodt
Sourced in United States
Ethyl acetate is a colorless, volatile liquid with a sweet, fruity odor. It is commonly used as a solvent in various industrial and laboratory applications due to its ability to dissolve a wide range of organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Mallinckrodt (3 mentions)
Acetone, by Mallinckrodt (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
β cryptoxanthin, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Citric acid, by Thermo Fisher Scientific (1 mentions)
Sodium benzoate, by Thermo Fisher Scientific (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
Chloroform, by Mallinckrodt (1 mentions)
Losartan, by Merck Group (1 mentions)
Tolcapone, by Merck Group (1 mentions)
Methyl pentadecanoate, by Merck Group (1 mentions)
Adomet, by Merck Group (1 mentions)
Hexane, by Mallinckrodt (1 mentions)
N n diisopropylethylamine, by Merck Group (1 mentions)
Plga 50 50, by Merck Group (1 mentions)
N boc ethylenediamine, by Merck Group (1 mentions)
Chitosan, by Thermo Fisher Scientific (1 mentions)
7 protocols using ethyl acetate
1
Deuterated Methanol and Solvents
2
Quantification of Bioactive Compounds
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Isosweet 100 high-fructose corn syrup was obtained from Tate & Lyle (Decatur, IL, USA), potassium sorbate from Spectrum (Gardena, CA, USA), sodium benzoate from Alfa Aesar (Heysham, Lancaster, UK), and sodium bicarbonate from Sigma Chemical Co. (St. Louis, MO, USA). Citric acid, mass spectrometry (MS) grade water, acetonitrile, methanol, formic acid, hydrochloric acid, and high pressure liquid chromatography (HPLC) grade acetone were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Chloroform and ethyl acetate were obtained from Mallinckrodt Chemicals (Phillipsburg, NJ, USA). CGA and FA were purchased from MP Biochemicals, LLC (Solon, OH, USA). All reagents were of analytical grade unless otherwise indicated.
3
Analysis of Methyltransferase Inhibitors
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AdoMet, S-(59-adenosyl)-L-homocysteine (AdoHcy), adenosine-29,39-dialdehyde (Adox), COMT, [ 13 C 16 ]-PA, tolcapone, 3,4-dihydroxyacetophenone (DHAP), 39-hydroxy-49-methoxyacetophenone (3H4MAP), 49-hydroxy-39-methoxyacetophenone (4H3MAP), N-tris(hydroxymethyl)-methyl-2-aminoethane sulphonic acid, methyl pentadecanoate, PAME, and losartan were purchased from Sigma-Aldrich (St. Louis, MO). Ethyl acetate, ethanol, and hexane were purchased from Mallinckrodt (St. Louis, MO).
4
Preparation of Biodegradable Polymer Nanoparticles
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PLGA 50:50, molecular weight 38,000 to 54,000 g/mol, polyvinyl alcohol (PVA) 87-89% hydrolyzed (31,000-50,000 g/mol), 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate), N-Boc-ethylenediamine, N,N-diisopropylethylamine, trifluoroacetic acid (TFA), triethylamine, TRITC and dichloromethane (DCM) were purchased from Sigma-Aldrich (MO, USA). Chitosan (MW 100,000-300,000 g/mol) was purchased from Fisher Scientific. Acetone, ethanol, ethyl acetate were HPLC grade (Mallinckrodt Baker, NJ, USA).
5
Antioxidant and Cytotoxicity Assay Protocol
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Methanol (MeOH), hydrochloric acid (HCl), sodium carbonate, sodium sulphate, acetone, phosphate buffered saline (PBS) were purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Folin-Ciocalteu reagent, quercetin, ascorbic acid, ferulic acid, chlorogenic acid, caffeic acid, p-coumaric acid and syringic acid, xanthophyll, zeaxanthin, β-cryptoxanthin, dichlorofluorescein- diacetate (DCFH-DA), were purchased from Sigma (St. Louis, MO). 2, 2-Azobis-amidinopropane (ABAP) was purchased from Wako Chemicals (Richmond, VA). Gallic acid was purchased from ICN Biomedical Inc. (Costa Mesa, CA). Ethyl acetate, triflouroacetic acid, and ethanol were purchased from Mallinckrodt (Paris, KS). Sodium hydroxide, hexane, acetonitrile, magnesium carbonate, tetrahydrofuran were obtained from Fisher Scientific (Pittsburgh, PA). MDA human breast cancer cell lines and HepG2 liver cancer cell lines are provided by the American Type Culture Collection (ATCC, Rockville, MD). Williams’ medium E (WME), α-MEM, Hanks’ Blanced Salt Solution (HBSS) were purchased from Gibco Life Technologies, and Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA).
6
Carotenoid Quantification in Biological Samples
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Lutein, β-cryptoxanthin, β-carotene, lycopene, ammonium acetate, butylatedhydroxytoluene (BHT), sodium hydroxide, potassium hydroxide, L-ascorbic acid, Na2-ethylenediaminetetraacetic acid, ethyl gallate, and HPLC-grade denatured ethanol were from Sigma-Aldrich (St. Louis, MO). Solvents including ethyl acetate, methanol, isopropyl alcohol, acetone, petroleum ether, hexanes and HCl were purchased from Mallinckrodt Baker (Phillipsburg, NJ, USA). Echinenone, α-cryptoxanthin and α-carotene were from CaroteNature (Lupsingen, Switzerland). Zeaxanthin was from IndoFine (Hillsborough, NJ).
7
Organic Solvent Extraction Protocol
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Ethyl acetate was ACS grade and was purchased from Mallinckrodt (Dublin, Ireland). Normal paraffin C7, C8, C9, C10 mix and C10, C12, C14, C16 mix were purchased from Aldrich (St. Louis, USA). Sodium chloride was purchased from Merck (Darmstadt, Germany). Tryptone (casein hydrolysate) enzymatic digest, yeast extract, and agar were purchased from USB Corporation (Cleveland, USA). All reagents were used as received without further purification.
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