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Ar 441 pan ar antibody

Manufactured by Santa Cruz Biotechnology
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The AR-441 pan-AR antibody is a laboratory research tool designed to detect androgen receptor (AR) protein expression in biological samples. It is a polyclonal antibody that recognizes multiple isoforms of the AR protein, making it a useful tool for studying AR expression in various cell and tissue types. The core function of this antibody is to provide a reliable and specific method for the detection and analysis of AR proteins in research applications.

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Lab products found in correlation

A1r confocal fluorescence microscope, by Nikon (2 mentions) Anti flag, by Merck Group (2 mentions) Alexa fluor 488 conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

AR antibody (10 citations) AR-441 antibody (5 citations)

2 protocols using ar 441 pan ar antibody

1

Immunofluorescence Quantification of AR-V7

Check if the same lab product or an alternative is used in the 5 most similar protocols
After fixation with 4% paraformaldehyde, cells were incubated with an anti-FLAG (for cumate-inducible AR-V7-expressing LNCaP cells; Sigma-Aldrich, F3165) or AR-441 pan-AR antibody (for PC-3 cells transfected with AR-V7; Santa Cruz Biotechnology, sc-7305) overnight at 4°C and then washed and incubated with Alexa Fluor 488-conjugated secondary antibody (Thermo Fisher, A-11001) for 1 hr at room temperature in the dark and counterstained with DAPI. An average of 10 fields were captured for each group using a Nikon A1r confocal fluorescence microscope. The intensity ratios of nuclear and cytoplasmic fluorescence signals were quantitated using ImageJ.
Roggero C.M., Jin L., Cao S., Sonavane R., Kopplin N.G., Ta H., Ekoue D.N., Witwer M., Ma S., Liu H., Ma T., Gioeli D., Raj G.V, & Dong Y. (2020). A detailed characterization of stepwise activation of the androgen receptor variant 7 in prostate cancer cells. Oncogene, 40(6), 1106-1117.
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2

Immunofluorescence Quantification of AR-V7

Check if the same lab product or an alternative is used in the 5 most similar protocols
After fixation with 4% paraformaldehyde, cells were incubated with an anti-FLAG (for cumate-inducible AR-V7-expressing LNCaP cells; Sigma-Aldrich, F3165) or AR-441 pan-AR antibody (for PC-3 cells transfected with AR-V7; Santa Cruz Biotechnology, sc-7305) overnight at 4°C and then washed and incubated with Alexa Fluor 488-conjugated secondary antibody (Thermo Fisher, A-11001) for 1 hr at room temperature in the dark and counterstained with DAPI. An average of 10 fields were captured for each group using a Nikon A1r confocal fluorescence microscope. The intensity ratios of nuclear and cytoplasmic fluorescence signals were quantitated using ImageJ.
Roggero C.M., Jin L., Cao S., Sonavane R., Kopplin N.G., Ta H., Ekoue D.N., Witwer M., Ma S., Liu H., Ma T., Gioeli D., Raj G.V, & Dong Y. (2020). A detailed characterization of stepwise activation of the androgen receptor variant 7 in prostate cancer cells. Oncogene, 40(6), 1106-1117.
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