Celltiter aqueous one solution reagent

Manufactured by Promega

Sourced in United States

The CellTiter AQueous One Solution Reagent is a colorimetric assay for determining the number of viable cells in a proliferation or cytotoxicity assay. The reagent contains a tetrazolium compound that is bioreduced by metabolically active cells, producing a colored formazan product that can be quantified using a spectrophotometer.

Automatically generated - may contain errors

Lab products found in correlation

Spectrophotometric microplate reader, by PerkinElmer (2 mentions) Synergy ht, by Agilent Technologies (1 mentions) H1n1 pr8 34 strain, by Charles River Laboratories (1 mentions) Incucyte flr live cell imaging system, by Sartorius (1 mentions) Cellplayer software module, by Sartorius (1 mentions) 96 pin woundmaker, by Sartorius (1 mentions) Imagelock plate, by Sartorius (1 mentions) 96 well plate, by Corning (1 mentions) Hcc827, by American Type Culture Collection (1 mentions) Calu 3, by American Type Culture Collection (1 mentions) Nci h1975, by American Type Culture Collection (1 mentions) Nci h1437, by American Type Culture Collection (1 mentions) Nci h441, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) reagent CellTiter 96® AQueous One Solution Reagent assay CellTiter 96® AQueous One Solution Reagent MTS assay (CellTiter 96 Aqueous One Solution reagent) CellTiter 96® Aqueous One Solution Cell Proliferation Reagent CellTiter 96 AQueous bromide One Solution Reagent CellTiter 96 AQueous One Solution Reagent for MTS assay CellTiter 96 AQueous One Solution Proliferation Assay reagent CellTiter 96R AQueous One Solution Reagent CellTiter 96® Aqueous One Solution Non-Radioactive (MTS) Cell Proliferation Assay Reagent

12 protocols using celltiter aqueous one solution reagent

Microglial Viability Assay using MTS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microglial viability was assessed by reduction of the tetrazolium compound (3-(4,5-dimethylthiazol-2-yl)-5-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS) contained in the CellTiter AQueous One Solution Reagent (Promega, Madison, WI, USA). For this assay cells were seeded in 96-well plates. At the end of the experimental procedure (46 h), 20 μL of MTS reagent were added to the cells that were further incubated for 2 h. Living cells bio-reduce yellow MTS into a purple soluble formazan product with an absorbance peak at 492 nm, that was read in a spectrophotometric microplate reader (PerkinElmer Inc, MA, USA).

Lisi L., Laudati E., Navarra P, & Dello Russo C. (2014). The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype. Journal of Neuroinflammation, 11, 125.

+ Open protocol

+ Expand

Cell Viability Assay for Particle Toxicity

Check if the same lab product or an alternative is used in the 5 most similar protocols

L929 fibroblasts and RAW 264.7 macrophages cells were seeded at 20000 cells per well in 96-well plates (Thermo Scientific Nunc Microwell Microplates, Waltham, MA) in phenol red free DMEM with 2% FBS and incubated at 37 °C, 5% CO₂, for 24 h before testing. Cells were incubated with particle suspensions at concentrations of 1, 0.5, 0.25, and 0.125 mg/mL in phenol red free DMEM with 2% FBS. Lived control wells received only medium, and lysed control wells were included. Following 2 and 48 h of incubation, the particles were removed and the cells were rinsed 3× with sterile Hank’s balanced salt solution (HBSS). Cell viability was then assayed by adding the CellTiter Aqueous One Solution reagent (MTS, Promega) directly to the culture wells and incubating for 2 h. Finally, the absorbance at 690 nm (background) and 490 nm (MTS assay) was recorded using a microplate reader (Synergy HT, BioTek Instruments, Inc., Winooski, VT). 690 nm readings from each well were subtracted from absorbance (OD) values at 490 nm, and the viability fraction was defined as

viability fraction = \frac{O D sample - O D lysed cells}{O D lived cells - O D lysed cells}

Durán-Lobato M., Carrillo-Conde B., Khairandish Y, & Peppas N.A. (2014). Surface-Modified P(HEMA-co-MAA) Nanogel Carriers for Oral Vaccine Delivery: Design, Characterization, and In Vitro Targeting Evaluation. Biomacromolecules, 15(7), 2725-2734.

+ Open protocol

+ Expand

Evaluating Cell Viability after Viral Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary mAECs, BMDMs or BMDCs were seeded into 48-well plates, grown to subconfluence, infected with H1N1 PR8/34 strain (Charles River) at 5 MOI, then incubated in the presence of DMSO, apoptosis or necroptosis pathway inhibitors for 24 or 48 h. Cell viability was assessed by MTS assay using CellTiter A_Queous one solution reagent according to the manufacturer's instructions (Promega, Madison, WI). Cell survival rate was calculated by comparison to DMSO-treated control cells and are presented as means ± SE (n = 3).

Wang Y., Hao Q., Florence J.M., Jung B.G., Kurdowska A.K., Samten B., Idell S, & Tang H. (2019). Influenza Virus Infection Induces ZBP1 Expression and Necroptosis in Mouse Lungs. Frontiers in Cellular and Infection Microbiology, 9, 286.

+ Open protocol

+ Expand

Microglial Viability Assessment via MTS Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microglial viability was assessed by reduction of the tetrazolium compound (3-(4,5-dimethylthiazol-2-yl)-5-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS) contained in the CellTiter AQueous One Solution Reagent (Promega, Madison, WI, USA). For this assay cells were seeded in 96-well plates. At the end of the experimental procedure, 20 μL of MTS reagent were added to the cells that were further incubated at 37 °C for 3 h. Living cells bio-reduce yellow MTS into a purple soluble formazan product with an absorbance peak at 492 nm that was read in a spectrophotometer plate reader (iMark, Biorad, Hercules, CA, USA).

Cappoli N., Mezzogori D., Tabolacci E., Coletta I., Navarra P., Pani G, & Dello Russo C. (2019). The mTOR kinase inhibitor rapamycin enhances the expression and release of pro-inflammatory cytokine interleukin 6 modulating the activation of human microglial cells. EXCLI Journal, 18, 779-798.

+ Open protocol

+ Expand

XTT Assay for Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

In the XTT assay, we used the CellTiter AQueous One Solution Reagent (Promega). The experiments were performed according to the instructions. Briefly, 5,000 cells in 100μL medium were seeded into a 96 well flat bottom plate in triplicate. Three control wells containing 100μL of complete growth medium only were set for blank absorbance readings. It usually took 12 hours for the cells to adhere. The medium was changed to RPMI1640 medium without FBS but with different concentration of H₂O₂ or TRAIL and incubated for indicated time intervals. Then the medium was changed with fresh RPMI1640 medium and 20μL of CellTiter AQueous One Solution Reagent was added directly to the well. The mixture was incubated for 2 hours and then the absorbance at 490nm was read with a 96 well plate reader.

Zhang F.F., Jiang C., Jiang D.P., Cui Y.Z., Wang X.Y., Sun L.Z., Chen M., Lam K.O., Wu S.Y., Verhoeft K., Kwong D.L, & Guan X.Y. (2023). ARHGAP15 promotes metastatic colonization in gastric cancer by suppressing RAC1-ROS pathway. PLOS Genetics, 19(2), e1010640.

+ Open protocol

+ Expand

Proliferation and Migration Assays for Prostate Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For proliferation assays, 5000 LNCaP cells or 3000 PC3 cells were seeded overnight into 96‐well plates (Corning, In vitro Technologies, Eight Mile Plains, Australia). Cells were washed in PBS, and fresh medium containing 1% FBS or 1% CSS was added. After 72 h, proliferation was assessed using CellTiter AQueous One Solution Reagent (Promega, Hawthorn, Australia). For migration assays, 20 000 LNCaP cells or 15 000 PC‐3 cells were seeded in a 96‐well Image‐lock plate (Essen BioScience Inc., Ann Arbor, MI, USA). Wounds were made through the monolayer of confluent cells using the 96‐pin WoundMaker (Essen BioScience Inc.) according to the manufacturer’s instructions. Wells were washed twice with PBS and fresh medium (RPMI + 1% FBS) was added before the initiation of imaging. Images were captured every 2 h for up to 72 h by the IncuCyte FLR live cell imaging system (Essen BioSciences Inc.). Wound closure kinetics were determined using the CellPlayer software module (Essen BioScience Inc.).

Kryza T., Bock N., Lovell S., Rockstroh A., Lehman M.L., Lesner A., Panchadsaram J., Silva L.M., Srinivasan S., Snell C.E., Williams E.D., Fazli L., Gleave M., Batra J., Nelson C., Tate E.W., Harris J., Hooper J.D, & Clements J.A. (2019). The molecular function of kallikrein‐related peptidase 14 demonstrates a key modulatory role in advanced prostate cancer. Molecular Oncology, 14(1), 105-128.

+ Open protocol

+ Expand

Photostimulation of Engineered Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary rat cortical neurons in 96 well plates were transduced with AAV vectors expressing iRFP713 or EYFP (4.4×10¹² viral genomes (vg)/mL and 4.2×10¹² vg/mL, respectively) on DIV6 by removing 100 μL of media from wells and adding 5 μL of virus for 2 hrs. Feeding protocol of 50% media exchange was repeated on DIV8 and DIV11. On DIV13 cells were fed and subjected to fluorescent light. Using a 40x magnification, cells transduced with AAV1-CamKIIa-EYFP were continuously exposed to FITC filtered light for 30 min at an intensity of 5.1mW (n=3). Similarly, cells transduced with AAV1-CaMKII-iRFP were exposed to CY5.5 filtered light with a light intensity of 12.5 mW for 30min (n=3). After light exposure, the cells were placed back into the incubator overnight. On DIV14, under sterile conditions, 100 μL of media was removed and 15 μL of CellTiter Aqueous One Solution Reagent (Promega) was added to each well to assess viability. Cells were placed back into the 37°C incubator for 1 hr. Using a plate reader, absorbance was read at 490nm.

Richie C.T., Whitaker L.R., Whitaker K.W., Necarsulmer J., Baldwin H.A., Zhang Y., Fortuno L., Hinkle J.J., Koivula P., Henderson M.J., Sun W., Wang K., Smith J.C., Pickel J., Ji N., Hope B.T, & Harvey B.K. (2017). Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies. Journal of neuroscience methods, 284, 1-14.

+ Open protocol

+ Expand

NSCLC Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human NSCLC cell lines including NCI-H1975, NCI-H1437, NCI-H441, HCC827, A549, and Calu-3 were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured in RPMI-1640 medium supplemented with 10% fetal calf serum. Primary normal human alveolar epithelial cells were from Cell Biologics (Chicago, IL, USA), cultured in epithelial cell growth medium, and used for experiments within four passages. For cell viability assay, cells were seeded into 48-well plates, grown to subconfluence, then incubated with various agonists in the presence or absence of Smac mimetics, caspase or kinases inhibitors for indicated time periods. Cell viability was assessed by MTS assay using CellTiter AQ_ueous one solution reagent according to the manufacturer’s instructions (Promega, Madison, WI, USA) and cell counting with trypan blue by a TC20 automated cell counter. Cell survival rate was calculated by comparison to DMSO-treated control cells and are presented as mean ± SE (n = 3).

Hao Q, & Tang H. (2018). Interferon-γ and Smac mimetics synergize to induce apoptosis of lung cancer cells in a TNFα-independent manner. Cancer Cell International, 18, 84.

+ Open protocol

+ Expand

Macrophage Viability Assessment by MTS Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The effect of poly(dA:dT) or poly(dG:dC) treatment on macrophage viability was measured by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium, inner salt (MTS) assay. Macrophages cultured in a 96-well plate (8×10⁴ cells/well) were transfected with poly(dA:dT) or poly(dG:dC) at different concentrations. The cells were cultured for 72 h before being treated with CellTiter AQueous One Solution Reagent (Promega, Madison, WI, USA) containing MTS and phenazine ethosulfate (20 μl/ml) was added to each well of the 96-well plate and then incubated for 4 h. Absorbance at 490 nm was measured after 4 h incubation.

Zhang B., Liu J.B., Zhou L., Wang X., Khan S., Hu W.H, & Ho W.Z. (2022). Cytosolic DNA Sensors Activation Inhibits HIV Infection of Macrophages. Journal of medical virology, 95(1), e28253.

+ Open protocol

+ Expand

Microglial Viability Assay by MTS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microglial viability was assessed by reduction of the tetrazolium compound MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] contained in the CellTiter AQueous One Solution Reagent (Promega). For this assay, cells were seeded in 96-well plates. At the end of the experimental procedure, 20 μl of MTS reagent were added to the cells that were further incubated for 2 h. Living cells bio-reduces yellow MTS into a purple soluble formazan product with an absorbance peak at 492 nm, that was read in a spectrophotometric microplate reader (PerkinElmer Inc.)

Lisi L., Stigliano E., Lauriola L., Navarra P, & Russo C.D. (2014). Proinflammatory-activated glioma cells induce a switch in microglial polarization and activation status, from a predominant M2b phenotype to a mixture of M1 and M2a/B polarized cells. ASN NEURO, 6(3), e00144.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!