18F-FDG was provided by the Department of Nuclear Medicine of Ruijin hospital affiliated Shanghai Jiao Tong University and the radiochemical purities were more than 95%. 18F-FDG PET/CT imaging was performed using a Micro-PET/CT scanner (Siemens; Siemens, Munich, Germany). The mice were not fed for at least for 6 h before 18F-FDG PET/CT imaging and 5.5 MBq (150 uCi) of 18F-FDG were injected into the tail vein 60 min before the scan start. Each mouse was then fixed in prone position at the midpoint of the visual filed on the scanning bed. One point five percent (1.5%) isoflurane with an O2 flow rate of 2 L/min was prepared and mice anesthetized during the scanning period. The mice were first subjected to n CT scan and then to PET scan. The 3D images were reconstructed using a three dimensional ordered-subset expectation maximum algorithm (PSEM3D). For data analysis, the region of interest (ROI) was automatic sketched out using the image segmentation algorithm to segment the tumor region in the PET functional image using the CT image. The maximum standardized uptake value (SUVmax) of the ROI was calculated. The SUV was calculated as [decay-corrected activity (kBq) per milliliter of tissue volume)/(injected 18F-FDG activity (kBq)/body mass (g)].
Micro pet ct scanner
Manufactured by Siemens
Sourced in Germany, United States
The Micro-PET/CT scanner is a laboratory imaging device that combines positron emission tomography (PET) and computed tomography (CT) technologies. It is designed to provide high-resolution, three-dimensional images of small animals or samples for research purposes.
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7 protocols using micro pet ct scanner
1
In Vivo 18F-FDG PET/CT Imaging Protocol
2
Radiolabeling and Purification of [18F]FDG
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All chemicals used in the synthesis were commercially sourced and used without further purification unless otherwise indicated. [18F]FDG was radiolabeled as previously described (Luo et al., 2009 (link)). Sep-Pak light QMA and Plus C18 cartridges were purchased from Waters Corporation (Milford, MA, USA). Sep-Pak light QMA cartridges were preconditioned with 10 ml of NaHCO3 aqueous (8.4%) and water in advance. Preconditioning of the Plus C18 cartridge was performed with 10 ml of ethanol followed by 10 ml of water. A micro PET/CT scanner by Siemens Healthineers (Erlangen, Germany) was used.
3
PET Imaging of Prefrontal Glucose Metabolism
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Prior to PET scans, mice were fasted for 12 h. Each mouse was injected with approximately 14.5–16.6 MBq of 18F FDG via the tail vein. After an uptake period of 40 min in a quiet and dimly-lit environment, each mouse was anaesthetized with 1% sodium pentobarbital (50 mg/kg, Beijing Chemical Reagent Company, Beijing, China), and was then placed in the prone position for imaging. PET signals and CT scans were acquired for 10 min in a micro-PET/CT scanner (Siemens, Germany). The raw PET data were binned into a single frame, and multiple planar reconstruction (MPR) images were obtained by the MAP reconstruction method. ASI Pro VM software ((https://www.siemens-healthineers.com/en-us/ , Siemens Medical Solutions, USA) was used for analysis and semi-quantification of the brain images in the region of interest (ROI), yielding the standardized uptake value (SUV) of the ROI that was representative of the metabolic rate in the corresponding regions. The prefrontal cortex was defined as the ROI. The prefrontal-cortex SUV was normalized to that of the whole-brain SUV within the same mouse, representing the level of glucose metabolism in the prefrontal cortex (normalized SUV values = prefrontal-cortex SUV/whole-brain SUV).
4
PET Imaging of Tumor Metabolism in Rats
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To assess the metabolism of tumour, four rats of each group were prepared for PET imaging 4 weeks after tumour cell injection. The animals were warmed and anesthetized throughout the entire imaging procedure, and then injected with 0.1 mCi 18F-FDG via a tail vein. After 1 h of unconscious uptake, images were acquired on a micro-PET/CT scanner (Siemens Inc., Erlangen, Germany) in the previous study [43 (link)]. The quantitative evaluation was performed by drawing 3D region of interests (ROIs). Maximum standardized uptake values (SUVmax) normalized for body weight and total radioactivity and metabolic tumour volume were calculated. After the PET scanning, the animals were sacrificed with an overdose of isoflurane on the next day and tumour tissues were retrieved for the following analysis.
5
Microstructural Analysis of Bone Metastases
6
Micro-PET/CT Imaging of 18F-GE180 and 18F-FDG in Rats
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Micro-PET/CT imaging was conducted as described previously using a micro-PET/CT scanner (Siemens Inc., USA) [21 (link), 22 (link)]. Briefly, static PET/CT imaging was collected for 20 min at 40 min post intravenous injection [14 (link)] of 18F-GE180 (296–444 MBq/kg body weight) and at 50 min post intravenous injection of 18F-FDG (370–555 MBq/kg body weight). Then, the rats were positioned in a spread-supine position on the imaging bed and anesthetized by 2–3% isoflurane in medical oxygen (1–2 L/min) at room temperature with an isoflurane vaporizer (Molecular Imaging Products Company, USA) during the PET/CT procedure. PET/CT images were reconstructed using the ordered subsets expectation maximization 3D algorithm, and data were reviewed using Inveon Research Workplace software (Siemens). GE180 imaging was performed on the last day of the drug treatment phase (day 6, defined as the drug addiction group) and the response assessment phase (day 12), while FDG imaging was only performed on day 6 to assist in the establishment of the GE180 template. One animal died during the GE180 imaging on day 6, and one died during CPA training, so the final sample size of DA, DW and CPA groups was 11, 6 and 5, respectively.
7
Micro-PET/CT Imaging in Drug Addiction
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Micro-PET/CT imaging was conducted as described previously using a micro-PET/CT scanner (Siemens Inc., United States) [18, 19] (link). Brie y, static PET/CT imaging was collected for 20 min at 40 min post intravenous injection [14] (link) of 18 F-GE180 (296-444 MBq/kg body weight) and at 50 min post intravenous injection of 18 F-FDG (370-555 MBq/kg body weight). Then, the rats were positioned in a spread-supine position on the imaging bed and anesthetized by 2-3% iso urane in medical oxygen (1-2 L/min) at room temperature with an iso urane vaporizer (Molecular Imaging Products Company, United States) during the PET/CT procedure. PET/CT images were reconstructed using the ordered subsets expectation maximization 3D algorithm, and data were reviewed using Inveon Research Workplace software (Siemens). GE180 imaging was performed on the last day of the drug treatment phase (day 6, de ned as the drug addiction group) and the response assessment phase (day 12), while FDG imaging was only performed on day 6 to assist in the establishment of the GE180 template. One animal died during the GE180 imaging on day 6, and one died during CPA training, so the nal sample size of DA, DW and CPA groups was 11, 6 and 5, respectively.
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