Annexin 5 binding apoptosis detection kit 2

The effect of Les-3331 and reference drug on the induction of apoptosis process in human breast cancer cell lines was evaluated using a flow cytometer (BD FACSCanto II, Becton Dickinson Biosciences Systems, San Jose, CA, USA) and Annexin V binding Apoptosis Detection Kit II (BD Biosciences, San Diego, CA, USA). The Les-3331 and reference drug were used in 1 µM and 5 µM concentrations. The test was carried out after 24 h incubation with the tested compounds according to the manufacturer’s protocol and was previously described by our research group [50 (link)]. Analysis of the obtained results was performed using FACSDiva software (version 6.1.3, BD Biosciences Systems, San Jose, CA, USA). The equipment was calibrated with BD Cytometer Setup and Tracking Beads (BD Biosciences, San Diego, CA, USA).

Flow cytometry assessment of Annexin V binding Apoptosis Detection Kit II (BD Biosciences, San Diego, CA, USA) was utilized to examine the induction of apoptosis in DLD-1 and HT-29 colorectal adenocarcinoma cell lines. The procedure of detection was carried out as maintained by manufacturer’s guidance. Tested compounds were added in 0.5 µM concentration. The experiment was realized exploited utilizing flow cytometer (BD FACSCanto II, Becton Dickinson Biosciences Systems, San Jose, CA, USA). Then received results were investigated with FACSDiva software (ver. 6.1.3, BD Biosciences Systems, San Jose, CA, USA). The assay was performed as described in the literature [46 (link)].

Flow cytometry assessment was performed using the Annexin V binding Apoptosis Detection Kit II (BD Biosciences, San Diego, CA, USA) to examine the induction of apoptosis in DLD-1 and HT-29 colorectal adenocarcinoma cell lines. The procedure of detection was done in accordance with the manufacturer’s guidelines. The assay was performed as described in the literature. The tested compounds were added in 1.5 and 3 µM concentrations. The experiment was done at flow cytometer (BD FACSCanto II, Becton Dickinson Biosciences Systems, San Jose, CA, USA). Then, the obtained results were investigated with FACSDiva software (version 6.1.3, BD Biosciences Systems, San Jose, CA, USA) [51 (link)].