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Folin ciocaleau s reagent

Manufactured by ITW Reagents
Sourced in United States

Folin-Ciocalteau's Reagent is a chemical solution used for the colorimetric determination of phenolic compounds. It consists of a mixture of phosphomolybdic and phosphotungstic acid complexes.

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2 protocols using folin ciocaleau s reagent

1

Analytical Methods for Material Characterization

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Sulfuric acid (H2SO4, 95.0–98.0%, ACS GR, Fluka, Charlotte, NC, USA), sodium hydroxide (NaOH, beads, PanReac, Barcelona, Spain), hydrogen peroxide (H2O2, 6% solution, ACS GR, VWR Chemicals, Radnor, PA, USA), Folin-Ciocaleau’s Reagent (1.8–2.2 mol/L, PanReac, Barcelona, Spain), sodium carbonate (NaCO3, ACS GR, PanReac, Barcelona, Spain), TPTZ (2,4,6-Tris (2-pyridil)-s-triazine, ≥99.0%, Sigma-Aldrich, St. Louis, MO, USA), chloridric acid (HCl, 36.5–38.0%, ACS GR, PanReac, Barcelona, Spain), iron (III) chloride hexahydrate (FeCl3·6H2O, ≥98%, Sigma-Aldrich, St. Louis, MO, USA), acetone ((CH3)2CO, ≥99.0%, VWR Chemicals, Radnor, PA, USA), ethanol (C2H5OH, 99.9%, VWR Chemicals, Radnor, PA, USA), methanol (CH3OH, ≥95%, Thermo Scientific, Waltham, MA, USA) were used as received without further purification. All water used throughout this work was purified by a Milli-Q water purification system (Barnstead™ Pacific TII Water, Thermo Scientific, Waltham, MA, USA).
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2

Analytical Methods for Reagent Characterization

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The reactants used in this study include hydrogen peroxide (H2O2, 6% solution, ACS GR) from VWR Chemicals (Radnor, PA, USA), chloridric acid (HCl, 36.5–38.0%, ACS GR), Folin–Ciocaleau’s Reagent (1.8–2.2 mol/L), sodium carbonate (NaCO3, ACS GR), and sodium hydroxide (NaOH, beads) from PanReac (Barcelona, Spain), sulfuric acid (H2SO4, 95.0–98.0%, ACS GR) from Fluka (Charlotte, NC, USA), TPTZ (2,4,6-Tris (2-pyridil)-s-triazine, ≥99.0%), and iron (III) chloride hexahydrate (FeCl3·6H2O, ≥98%) from Sigma-Aldrich (St. Louis, MO, USA).
The solvents, used as received without further purification, include acetone ((CH3)2CO, ≥99.0%), ethanol (C2H5OH, 99.9%) from VWR Chemicals, and methanol (CH3OH, ≥95%) from Thermo Scientific (Waltham, MA, USA).
Milli-Q water (Barnstead™ Pacific TII Water, Thermo Scientific) throughout this study.
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