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Cc 2571

Manufactured by Lonza
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Sourced in Switzerland

The CC-2571 is a compact and versatile laboratory centrifuge designed for a range of general-purpose applications. It features a maximum speed of 6,000 rpm and a maximum relative centrifugal force (RCF) of 4,020 x g. The CC-2571 accommodates a variety of sample containers, including tubes and microplates. Its user-friendly control panel allows for easy program selection and operation.

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Lab products found in correlation

Cc 3182, by Lonza (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Cc 2535, by Lonza (2 mentions) Cc 7014, by Lonza (2 mentions) Fetal bovine serum (fbs), by Lonza (2 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions) Cc 3181, by Lonza (1 mentions) 213 ilb, by R&D Systems (1 mentions) Cc 3162, by Lonza (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Fibroblast growth factor (fgf), by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mcf 10a, by American Type Culture Collection (1 mentions) Bt549, by American Type Culture Collection (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Bt474, by American Type Culture Collection (1 mentions) Hs578t, by American Type Culture Collection (1 mentions)

11 protocols using cc 2571

1

Aortic Smooth Muscle Cell Stretch Assay

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Commercial primary human aortic smooth muscle cells (HAoSMCs) from one male donor with no reported cardiovascular comorbidities were obtained from Lonza (#CC-2571, Lot No. 0000369150, ascending aorta, Basel, Switzerland) and subjected to 0% or 12.5% substrate stretch as previously described [21 (link)]. In brief, custom made silicon chambers (inner dimensions: 4 cm2 or 3 cm2) with replaceable silicon membrane bottoms were sterilized and coated with human fibronectin (5 μg/cm2, #PHE0023, Life Technologies, Carlsbad, CA, USA). HAoSMCs at passage 6 were plated at sub-confluence (50,000 cells per cm2) and left to adhere overnight in basal growth medium (5% FBS, #CC-3181, Lonza, Basel, Switzerland). After 4 h of serum starvation in OptiMEM medium containing 0.1% FBS (#51985-026, Life Technologies, Carlsbad, CA, USA), the medium was changed to OptiMEM medium containing 1% FBS and 1.5 mM Ca, 1.5 mM PO4. Controls were treated identically and supplement volumes replaced with PBS. Axial cyclic stretch was applied to the silicone chambers with a frequency of 70 cycles per minute. For comparison, HAoSMCs were also plated in fibronectin coated regular 6-well plates for expression analysis and chamber slides for immunofluorescence (IF) imaging, under identical medium conditions. After 8, 24 and 48 h medium supernatant, cell lysates or complete membranes were collected for further analysis.
Seime T., van Wanrooij M., Karlöf E., Kronqvist M., Johansson S., Matic L., Gasser T.C, & Hedin U. (2022). Biomechanical Assessment of Macro-Calcification in Human Carotid Atherosclerosis and Its Impact on Smooth Muscle Cell Phenotype. Cells, 11(20), 3279.
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2

Aortic Smooth Muscle Cell Uremic Serum Study

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Human aortic smooth muscle cells were obtained from Lonza (CC-2571)and cultured in smooth muscle basal media (SmBM), supplemented with growth factors and 5% FBS (CC-3182), according to the manufacturer’s guidelines. Cells from passage 4-8 were used in the described studies. Throughout the studies, cells from different lots were used. For experimental conditions, growth medium was supplemented with 10% vol/vol normal or uremic serum. We used pooled serum from (3 (link)-5 ) maintenance hemodialysis patients in each experiment. Experiments were repeated using serum from different hemodialysis patients. Normal pooled serum from healthy donors was used for comparison in the different assays.
Monroy M.A., Fang J., Li S., Ferrer L., Birkenbach M.P., Lee I.J., Wang H., Yang X.F, & Choi E.T. (2015). Chronic kidney disease alters vascular smooth muscle cell phenotype. Frontiers in bioscience (Landmark edition), 20, 784-795.
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3

VSMC Responses to IL-13 Exposure

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Human vascular smooth muscle cells (VSMCs, CC-2571; Lonza, Visp, Switzerland) were grown in endothelial cell growth medium (EGM)-2 plus bullet kit media (CC-3162; Lonza). Human retinal endothelial cells (HRECs, ACBRI181; Cell Systems, Kirkland, WA) were grown in CSC complete recombinant media (SF-4Z0; Cell Systems). To study the role of VSMCs, they were exposed to or not exposed to 2, 10, and 50 ng/ml of recombinant interleukin (IL)-13 (213-ILB; R&D Systems, Minneapolis, MN). After incubating for 24 h, the total RNA was extracted using TRIzol reagent (Life Technologies). After a 72-h incubation, the supernatant was collected and the lysated protein was extracted with lysis buffer.
Kobayashi Y., Yoshida S., Zhou Y., Nakama T., Ishikawa K., Arima M., Nakao S., Sassa Y., Takeda A., Hisatomi T., Ikeda Y., Matsuda A., Sonoda K.H, & Ishibashi T. (2016). Tenascin-C promotes angiogenesis in fibrovascular membranes in eyes with proliferative diabetic retinopathy. Molecular Vision, 22, 436-445.
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4

Culturing Human Aortic Smooth Muscle Cells

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Smooth muscle cells derived from human aorta, AoSMC (CC-2571, LONZA, Colmar, France) were grown (37 °C, 10% CO2) in smooth muscle complete cell growth medium (CC-3182, LONZA, Colmar, France) containing SmBMTM Basal Medium and SmGMTM 2 SingleQuots supplements. Supplements comprise 5% (v/v) of fetal bovine serum (FBS), 0.1% (v/v) insulin, 0.2% (v/v) of human basic fibroblast growth factor (hFGF-B), 0.1% (v/v) of gentamicin, 0.1% (v/v) of amphotericin-B, and 0.1% (v/v) of human epidermal growth factor (hEGF). All experiments were conducted using actively growing cells at around 90% of confluency.
El Hajj S., Canabady-Rochelle L., Fries-Raeth I, & Gaucher C. (2024). A Smooth Muscle Cell-Based Ferroptosis Model to Evaluate Iron-Chelating Molecules for Cardiovascular Disease Treatment. Current Issues in Molecular Biology, 46(2), 1348-1359.
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5

Culturing Human Aortic Smooth Muscle Cells

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Human aortic SMCs were obtained from Lonza (CC-2571). The SMCs were cultured up to passage 6 in M199 medium supplemented with 10% FBS and growth factors (PeproTech, EGF and FGF).
Saito J., Dave J.M., Lau F.D, & Greif D.M. (2023). Presenilin-1 in smooth muscle cells facilitates hypermuscularization in elastin aortopathy. iScience, 27(1), 108636.
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6

Breast Cancer Cell Line Characterization and Maintenance

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The HMLE, MCF10A, MCF-7, MDA-MB-231, MDA-MB-468, BT-474, BT-549, SUM-159, Hs578T, T-47D, 293T, and HeLa cells were from American Type Culture Collection (ATCC; Manassas, VA, USA) and were maintained in low passage (< 15) for this study. ATCC validates cell lines by short tandem repeat profiles that are generated by simultaneous amplification of multiple short tandem repeat loci and amelogenin (for gender identification). The cell lines, with the exception of HMLE and MCF10A, were cultured in Dulbecco’s modified eagle’s medium (DMEM; Thermo Scientific, Waltham, MA, USA) containing 10% FBS and penicillin and streptomycin (Mediatech, Manassas, VA, USA) at 37°C in a humidified atmosphere of 5% CO2. HMLE and MCF10A cell lines were cultured in defined mammary epithelial growth media (CC-2571, MEGM Bullet Kit, Lonza, Inc., Basel, Switzerland) supplemented with bovine pituitary extracts and epidermal growth factor.
Kim J.H., Sharma A., Dhar S.S., Lee S.H., Gu B., Chan C.H., Lin H.K, & Lee M.G. (2014). UTX and MLL4 Coordinately Regulate Transcriptional Programs for Cell Proliferation and Invasiveness in Breast Cancer Cells. Cancer research, 74(6), 1705-1717.
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7

Investigating NF-κB Signaling in Smooth Muscle Cells

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Human pulmonary artery (hPASMC) (Lonza CC-2581) from three commercial donors and four primary donors, including those with and without disease-related BMPR2 mutations, and human aortic smooth muscle cells (hAoSMC) (Lonza CC-2571) from two donors were maintained in smooth muscle cell media SmGM-2 including bullet kit supplements (Lonza CC-3182). All experiments were performed between passages 4 and 8. Quiescent media (Dulbecco’s modified Eagles medium (Lonza 12-604) with 0.2% (v/v) fetal bovine serum ((FBS) Lonza 14-401) was added for 48 h prior to transfection with siRNA/reporter plasmid (Qiagen Cignal NFkB luciferase reporter) and/or 6-h stimulation with IL-1ß at 10 ng/mL or BMP4 at 20 ng/mL.13 (link),14 (link)
Pickworth J., Rothman A., Iremonger J., Casbolt H., Hopkinson K., Hickey P.M., Gladson S., Shay S., Morrell N.W., Francis S.E., West J.D, & Lawrie A. (2017). Differential IL-1 signaling induced by BMPR2 deficiency drives pulmonary vascular remodeling. Pulmonary Circulation, 7(4), 768-776.
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8

Cell Culture of Vascular and Immune Cells

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HAECs (CC-2535) from a 54-year-old male and HASMCs (CC-2571) from a 22-year-old male were purchased from Lonza. HAECs and HASMCs were cultured in EC growth media-2 (CC-3202, Lonza) and SMC growth medium-2 (C-22062, Promo Cell), respectively, at 37°C, 5% CO2, in a humidified cell culture incubator. Both HAECs and HASMCs were used from passages 4 to 8 in all experiments. The human monocyte cell line THP-1 was purchased from ATCC and grown in RPMI 1640 containing 10% FBS (Thermo Fisher Scientific) and 50 mg/mL of a penicillin/streptomycin solution.
Zhao G., Chang Z., Zhao Y., Guo Y., Lu H., Liang W., Rom O., Wang H., Sun J., Zhu T., Fan Y., Chang L., Yang B., Garcia-Barrio M.T., Chen Y.E, & Zhang J. (2021). KLF11 protects against abdominal aortic aneurysm through inhibition of endothelial cell dysfunction. JCI Insight, 6(5), e141673.
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9

Gene Expression Profiling of Vascular Cells

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ADAMTS7 and CHRNB4-A3-A5 mRNA levels were measured in cultured human coronary artery smooth muscle cells (HCASMC; Lonza CC-2583, Lonza Walkersville, MD), human coronary artery endothelial cells (HCAEC, Lonza CC-2585), human aortic smooth muscle cells (HAoSMC, Lonza CC-2571), human aortic endothelial cells (HAoEC, Lonza CC-2535), human aortic adventitial fibroblasts (HAoAF, Lonza CC-7014), and human acute monocytic leukemia cell line (THP-1, ATCC TIB-202). Further details are provided in eMethods.
Saleheen D., Zhao W., Young R., Nelson C.P., Ho W., Ferguson J.F., Rasheed A., Ou K., Nurnberg S.T., Bauer R.C., Goel A., Do R., Stewart A.F., Hartiala J., Zhang W., Thorleifsson G., Strawbridge R.J., Sinisalo J., Kanoni S., Sedaghat S., Marouli E., Kristiansson K., Zhao J.H., Scott R., Gauguier D., Shah S.H., Smith A.V., van Zuydam N., Cox A.J., Willenborg C., Kessler T., Zeng L., Province M.A., Ganna A., Lind L., Pedersen N.L., White C.C., Joensuu A., Kleber M.E., Hall A.S., März W., Salomaa V., O’Donnell C., Ingelsson E., Feitosa M.F., Erdmann J., Bowden D.W., Palmer C.N., Gudnason V., De Faire U., Zalloua P., Wareham N., Thompson J.R., Kuulasmaa K., Dedoussis G., Perola M., Dehghan A., Chambers J.C., Kooner J., Allayee H., Deloukas P., McPherson R., Stefansson K., Schunkert H., Kathiresan S., Farrall M., Frossard P.M., Rader D.J., Samani N.J, & Reilly M.P. (2017). Loss of Cardio-Protective Effects at the ADAMTS7 Locus Due to Gene-Smoking Interactions. Circulation, 135(24), 2336-2353.
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10

Cell Culture of Vascular Smooth Muscle

Cited 1 time
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HASMCs (CC-2571) from a 22-year-old male were purchased from Lonza and cultured in SMC growth medium 2 (Lonza, CC-3182) containing 5% fetal bovine serum (Lonza) and 1% penicillin/streptomycin solution (Gibco, Thermo Fisher Scientific; 15140122) at 37°C, 5% CO2 in a humidified cell culture incubator. HASMCs were used from passages 4 to 8 in all experiments. The rat embryonic thoracic aortic SMC line A7r5 was purchased from ATCC and cultured in DMEM/Nutrient Mixture F-12 (DMEM/F12) (Gibco, Thermo Fisher Scientific; 11320-033) containing 10% FBS (Thermo Fisher Scientific) and 50 mg/mL of a penicillin/streptomycin solution. Mouse BMDMs were obtained as previously described (57 (link)).
Zhao G., Zhao Y., Lu H., Chang Z., Liu H., Wang H., Liang W., Liu Y., Zhu T., Rom O., Guo Y., Chang L., Yang B., Garcia-Barrio M.T., Lin J.D., Chen Y.E, & Zhang J. (2022). BAF60c prevents abdominal aortic aneurysm formation through epigenetic control of vascular smooth muscle cell homeostasis. The Journal of Clinical Investigation, 132(21), e158309.
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