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Cholesterol

Manufactured by Randox
Sourced in United Kingdom

The Cholesterol lab equipment product is designed to quantitatively measure the concentration of cholesterol in a given sample. It provides accurate and reliable results to support clinical diagnostic testing and research applications.

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3 protocols using cholesterol

1

Serum Biomarker Analysis Protocol

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Serum analysis used commercial kits according to the manufacturer’s instructions; serum albumin (Alb), bilirubin and alanine transaminase (ALT) (Alpha Laboratories); aspartate aminotransferase (AST) and alkaline phosphatase(ALP) (Randox laboratories); Cholesterol (Wako Chemicals GmbH). All kits were adapted for use on a Cobas Fara centrifugal analyser (Roche Diagnostics).
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2

Comprehensive Metabolic Profiling Protocol

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Blood glucose was measured by glucometer (Accu-Chek). Plasma insulin was measured using ultrasensitive ELISA kit (Alpco, Salem, USA); C-peptide was determined using ELISA kit (Crystal Chem, Elk Grove Village, USA). Plasma triglyceride (TG) (Randox Laboratories, Crumlin, UK), non-esterified fatty-acids (NEFA) (Wako Chemicals, Richmond, VA), cholesterol (Randox Laboratories, Crumlin, UK) and free glycerol levels (Sigma–Aldrich) were enzymatically determined according to the manufacturer's instructions.
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3

Comprehensive Metabolic Profiling in Mice

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Insulinemia was measured using the Ultrasentitive ELISA kit (Alpco 80-INSMSU-E01, Salem NH). Hepatic triglycerides (TG) were extracted in methanol/chloroform.26 (link) Plasma and hepatic TG were analyzed with Triglycerides Reagent kit (Randox Laboratories, UK). Cholesterol (Randox Laboratories, UK), nonesterified fatty acids (NEFA) (Thermo Scientific, USA) and leptin (Mouse/Rat Leptin Quantikineࣨ ELISA Kit, R&D Systems) were also measured in plasma samples. Alanine (ALT) and aspartate (AST) aminotransferases plasma levels were determined by biochemical analyses at the Quebec Heart and Lung Institute clinical biochemistry department. To determine cytokine concentrations in liver, proteins from 75 mg of tissue were extracted in PBS 1X buffer (Igepal 1% and proteinase inhibitors 1X). Proteins were measured (Pierce BCA Protein Bioassay kit) and standardized for the multiplex assay, which was performed according to the manufacturer’s instructions (Biorad). Analysis of FA profile in liver was achieved at CHU Sainte-Justine Research Center.27 (link)
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