Ab7842

Manufactured by Cell Signaling Technology

Ab7842 is a primary antibody produced by Cell Signaling Technology. It is designed for use in Western blotting applications.

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Lab products found in correlation

Lsm 880 confocal microscope, by Zeiss (2 mentions) Ab8925, by Abcam (1 mentions) Ab62484, by Abcam (1 mentions) Ab47267, by Abcam (1 mentions) G2654, by Abcam (1 mentions) Ab15580, by Abcam (1 mentions) Gtvision detection system mo rb, by Gene Tech (1 mentions) Hoechst, by Thermo Fisher Scientific (1 mentions) Mab3299, by Merck Group (1 mentions) Anti glucagon, by Cell Signaling Technology (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Jackson ImmunoResearch (1 mentions)

4 protocols using ab7842

Pancreas Tissue Immunohistochemistry and Immunofluorescence

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Pancreas tissues were separated, fixed, and embedded in paraffin [21 (link)]. Serial sections were deparaffinized with dimethylbenzene, and antigen retrieval was conducted using citrate buffer solution. The slides were incubated with 3% hydrogen peroxide for 15 min and then blocked with 10% goat serum for 1 hour at room temperature. The slides were incubated with primary antibodies targeting Ngn3 (AVIVA SYSTEMS BIOLOGY, OAAB15617), cleaved Notch1 (Abcam, ab8925), insulin (Abcam, ab7842), NKX6-1 (Cell Signaling Technology, #54551), glucagon (Sigma, G2654), PDX-1 (Abcam, ab47267), Ki67 (Abcam, ab15580), and caspase12 (Abcam, ab62484) at 4°C in a refrigerator overnight. For immunohistochemistry, the GTVision™+ Detection System/Mo&Rb (Gene Tech, GK6005) was used to examine the signal based on the guidelines. For immunofluorescence staining, the signal was detected using FITC-labelled IgG fluorescence.

Yuan F., Wang D., Ma L., Qin X., Gong J., Hu M., Su H., Dong H, & Lu F. (2019). Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic β Cell Identity in db/db Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 1232913.

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Immunohistochemical Analysis of Pancreatic Tissues

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Using formalin-fixed paraffin embedded pancreatic tissue, 5μm sections were cut for immunohistochemistry analysis as described (29 (link)). Initial analysis was performed using DAB staining to quantify total pancreas area, islet fraction, and insulin positive area. For insulin positive area measurements of IHC-stained pancreas sections, whole slide images were analyzed in Visiopharm (Visiopharm, Høsersholm, Denmark) software using custom pipelines to identify pancreas tissue and DAB-positive regions. Islet architecture was examined using primary antibodies for insulin (Abcam # ab7842) and glucagon (Cell Signaling #2760). All primary antibody detection was performed using Alexa Fluor secondary conjugation (Alexa 488, Alexa Fluor Plus 555).

HUANG T.Y., GOLDSMITH F.R., FULLER S.E., SIMON J., BATDORF H.M., SCOTT M.C., ESSAJEE N.M., BROWN J.M., BURK D.H., MORRISON C.D., BURKE S.J., COLLIER J.J, & NOLAND R.C. (2019). Response of Liver Metabolic Pathways to Ketogenic Diet and Exercise Are Not Additive. Medicine and Science in Sports and Exercise, 52(1), 37-48.

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Quantitative Pancreatic Immunohistochemistry

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Using formalin-fixed paraffin embedded pancreatic tissue, 5-μm sections were cut for immunohistochemistry analysis as described (29 (link)). Initial analysis was performed using DAB staining to quantify total pancreas area, islet fraction, and insulin positive area. For insulin positive area measurements of immunohistochemistry-stained pancreas sections, whole slide images were analyzed in Visiopharm (Visiopharm, Høsersholm, Denmark) software using custom pipelines to identify pancreas tissue and DAB-positive regions. Islet architecture was examined using primary antibodies for insulin (Abcam ab7842) and glucagon (Cell Signaling 2760). All primary antibody detection was performed using Alexa Fluor secondary conjugation (Alexa 488; Alexa Fluor Plus 555).

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Multiparametric Immunofluorescence Analysis

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Paraffin embedded tissue sections were blocked with 5% normal goat serum for 1 h, and then incubated overnight at 4 °C with primary antibodies as follows: anti-Insulin (1:100, Abcam, ab7842), anti-Glucagon (1:150, Cell Signaling Technology, 2760S), anti-CD8 (1:100, Bio-Rad, MCA48GA), anti-CD68 (1:100, Bio-Rad, MCA341GA), anti-DNA (1:20, Millipore, single stranded specific, MAB3299). Secondary antibodies were employed as follows: Alexa Fluor 488 (1:400, Jackson ImmunoResearch or Thermo Fisher Scientific) and Alexa Fluor 594 (1:400, Thermo Fisher Scientific). Nuclei were stained with Hoechst (1:1000, Thermo Fisher Scientific, H3570). Images were acquired via ZEISS LSM 880 confocal microscope, and data collection was performed using ZEN 2.1 SP2 Black version 13.0.2.518 (ZEISS). The statistical analysis of fluorescent images was performed independently and blindly by two individuals.

Zhao J.M., Su Z.H., Han Q.Y., Wang M., Liu X., Li J., Huang S.Y., Chen J., Li X.W., Chen X.Y., Guo Z.L., Jiang S., Pan J., Li T., Xue W, & Zhou T. (2024). Deficiency of Trex1 leads to spontaneous development of type 1 diabetes. Nutrition & Metabolism, 21, 2.

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