Lymphocytes were isolated from cranial bone marrow using a previously reported protocol37 (link). In brief, the calvaria was cut into small pieces using sterile scissors and dissociated in PBS + 2% FBS with a pestle. Spleens were harvested from mice, washed in PBS and cut into 0.5 mm cubes in ice-cold PBS. Spleen or bone marrow isolates were transferred to a 70 μm cell strainer (Falcon, catalog no. 352350); cells were washed with PBS and resuspended in red blood cell lysis buffer (BioLegend, catalog no. 420301). Cells were stained for 30 min on ice using the following antibodies: anti-CD3 BV711 or anti-CD3-AF647 (clone okt3, BD Biosciences, catalog nos. 750983 and 566686)), anti-MuSK PE (clone 189-1 or 24C10), antihuman Ig light chain λ PE (clone MHL-38, BioLegend, catalog no. 316608), antihuman Ig light chain κ APC (clone MHK-49, BioLegend, catalog no. 316510), antihuman IgG PE (BD Biosciences, catalog no. 555787) and/or antimouse IgG APC (clone A85-1, BD Biosciences).
Antimouse igg apc clone a85 1
Manufactured by BD
Antimouse IgG APC (clone A85-1) is a laboratory reagent used for flow cytometric analysis. It is a fluorescently labeled antibody that binds to mouse immunoglobulin G (IgG) molecules, allowing the detection and quantification of mouse IgG in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Red blood cell lysis buffer, by BioLegend (2 mentions)
Anti cd3 bv711 or anti cd3 af647 clone okt3, by BD (2 mentions)
Anti musk pe clone 189 1 or 24c10, by BioLegend (2 mentions)
Antihuman ig light chain λ pe clone mhl 38, by BioLegend (2 mentions)
Antihuman ig light chain κ apc clone mhk 49, by BioLegend (2 mentions)
Anti human igg pe, by BD (2 mentions)
70 μm cell strainer, by BD (2 mentions)
2 protocols using antimouse igg apc clone a85 1
1
Isolation and Characterization of Lymphocytes from Bone Marrow and Spleen
2
Isolation and Characterization of Lymphocytes from Bone Marrow and Spleen
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Lymphocytes were isolated from cranial bone marrow using a previously reported protocol37 (link). In brief, the calvaria was cut into small pieces using sterile scissors and dissociated in PBS + 2% FBS with a pestle. Spleens were harvested from mice, washed in PBS and cut into 0.5 mm cubes in ice-cold PBS. Spleen or bone marrow isolates were transferred to a 70 μm cell strainer (Falcon, catalog no. 352350); cells were washed with PBS and resuspended in red blood cell lysis buffer (BioLegend, catalog no. 420301). Cells were stained for 30 min on ice using the following antibodies: anti-CD3 BV711 or anti-CD3-AF647 (clone okt3, BD Biosciences, catalog nos. 750983 and 566686)), anti-MuSK PE (clone 189-1 or 24C10), antihuman Ig light chain λ PE (clone MHL-38, BioLegend, catalog no. 316608), antihuman Ig light chain κ APC (clone MHK-49, BioLegend, catalog no. 316510), antihuman IgG PE (BD Biosciences, catalog no. 555787) and/or antimouse IgG APC (clone A85-1, BD Biosciences).
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