E. coli BL21 transformed with pTrcHisB-GLD-2 were cultured in LB medium containing 100 μg/ml ampicillin. Cells were cultured at 37°C to 0.8 OD at 600 nm, and protein expression was induced at 25°C by the addition of 1 mM isopropyl-β-
Ni nitrilotriacetic acid agarose resin
Ni-nitrilotriacetic acid agarose resin is a chromatography media used for the purification of recombinant proteins with a histidine-tag. It utilizes the high-affinity interaction between nickel ions and the histidine residues to capture and isolate the target proteins.
Lab products found in correlation
3 protocols using ni nitrilotriacetic acid agarose resin
Purification of GLD-2 Protein from E. coli
E. coli BL21 transformed with pTrcHisB-GLD-2 were cultured in LB medium containing 100 μg/ml ampicillin. Cells were cultured at 37°C to 0.8 OD at 600 nm, and protein expression was induced at 25°C by the addition of 1 mM isopropyl-β-
Recombinant Expression and Purification of SirT6 and SirT7
Preparation of recombinant histones were performed from E. coli Bl21 (DE3) cells as described elsewhere (48 (link)). Histone octamers were concentrated to 3 to 15 mg/ml, adjusted to 50% (v/v) glycerol, and stored at −80°C.
Recombinant Influenza Protein Production
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