Rat osteocalcin immunoassay kit

Manufactured by Thermo Fisher Scientific

The Rat Osteocalcin Immunoassay Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure rat osteocalcin levels in serum, plasma, and cell culture supernatants.

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Lab products found in correlation

Ratlaps enzyme immunoassay kit, by Immunodiagnostic Systems (3 mentions)

Spelling variants of this product

Osteocalcin (11 citations) Rat Osteocalcin Enzyme Immunoassay Kit (4 citations)

3 protocols using rat osteocalcin immunoassay kit

Serum Biomarkers of Bone Metabolism

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Serum C-terminal telopeptide of type I collagen (CTX) levels were measured using a RatLapsTM enzyme-immunoassay kit from Immunodiagnostic Systems (Fountain Hills, AZ). Serum concentrations of osteocalcin were measured using a rat osteocalcin immunoassay kit (Alfa Aesar). Serum levels of irisin were determined using a rat irisin competitive ELISA kit (Adipogen). All samples were assayed in duplicate, following the manufacturer’s protocols.

Zhao W., Peng Y., Hu Y., Guo X.E., Li J., Cao J., Pan J., Feng J.Q., Cardozo C., Jarvis J., Bauman W.A, & Qin W. (2020). Electrical Stimulation of Hindlimb Skeletal Muscle has Beneficial Effects on Sublesional Bone in a Rat Model of Spinal Cord Injury. Bone, 144, 115825.

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Bone Remodeling Marker Quantification

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Serum C‐terminal telopeptide of type I collagen (CTX) levels were measured using a RatLaps^TM enzyme‐immunoassay kit from Immunodiagnostic Systems (Fountain Hills, AZ, USA). Serum levels of P1NP were determined using a rat P1NP ELISA kit (MyBioSource). Serum concentrations of osteocalcin were measured using a rat osteocalcin immunoassay kit (Alfa Aesar). All samples were assayed in duplicate, following the manufacturer's protocols.

Peng Y., Langermann S., Kothari P., Liu L., Zhao W., Hu Y., Chen Z., Moraes de Lima Perini M., Li J., Cao J., Guo X.E., Chen L., Bauman W.A, & Qin W. (2023). Anti‐Siglec‐15 Antibody Prevents Marked Bone Loss after Acute Spinal Cord Injury‐Induced Immobilization in Rats. JBMR Plus, 7(12), e10825.

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Serum Biomarkers of Bone Turnover

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Blood samples collected at termination were centrifuged at 1500 g for 10 min at 4 °C, and the serum was separated and then stored at −80 °C. Serum C-terminal telopeptide of type I collagen (CTX) levels were measured using a RatLapsTM enzyme-immunoassay kit from Immunodiagnostic Systems (Fountain Hills, AZ). Serum concentrations of osteocalcin were measured using a rat osteocalcin immunoassay kit (Alfa Aesar). All samples were assayed in duplicate, following the manufacturer’s recommended procedures, as previously described [22 (link), 37 (link)].

Zhao W., Li X., Peng Y., Qin Y., Pan J., Li J., Xu A., Ominsky M.S., Cardozo C., Feng J.Q., Ke H.Z., Bauman W.A, & Qin W. (2018). Sclerostin Antibody Reverses the Severe Sublesional Bone Loss in Rats After Chronic Spinal Cord Injury. Calcified tissue international, 103(4), 443-454.

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