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P s6 s235 6

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P-S6 (S235/6) is a monoclonal antibody that specifically recognizes the phosphorylated form of the ribosomal protein S6 at serine residues 235 and 236. This antibody can be used to detect the activation of the mTOR signaling pathway in biological samples.

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Lab products found in correlation

Cleaved parp, by Cell Signaling Technology (2 mentions) Pan akt, by Cell Signaling Technology (2 mentions) β tubulin antibody, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Mg132, by Merck Group (2 mentions) Bafilomycin a1, by Merck Group (2 mentions) Rapamycin, by Merck Group (2 mentions) Puromycin, by Merck Group (2 mentions) Hygromycin b, by Corning (2 mentions) Mcl 1, by Cell Signaling Technology (2 mentions) Pras40, by Cell Signaling Technology (2 mentions) C flip antibody, by Enzo Life Sciences (2 mentions) Hexadecylsulfonylfluoride hdsf, by Santa Cruz Biotechnology (2 mentions) Rag b, by Cell Signaling Technology (2 mentions) Tw 37, by Selleck Chemicals (2 mentions) C myc, by Santa Cruz Biotechnology (2 mentions) Cell culture supplies, by Thermo Fisher Scientific (1 mentions) Jnk in 8, by Merck Group (1 mentions) Crt0066101, by Merck Group (1 mentions)

3 protocols using p s6 s235 6

1

Endothelin-1 Signaling Pathway Analysis

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Chemicals were purchased from Sigma‐Aldrich unless otherwise stated. Cell culture supplies were purchased from Life Technologies. Endothelin 1 (CSCSSLMDKECVYFCHLDIIW) was purchased from Bachem (Product No. 4040254) and is referred to as EDN for the purpose of all stimulation experiments. The following key reagents were employed in the study: TiO2 (GL Sciences), 13C6‐15N2 lysine (Lys‐8, Silantes GmbH) and 13C6‐15N4 arginine (Arg‐10, Silantes GmbH). Heavy peptides for SRM were synthesised by JPT Peptide Technologies. Kinase inhibitors were purchased from Selleckchem, except for ddcAMP (Enzo Life Sciences), GSK‐429286, CRT0066101 and JNK‐IN‐8 (Sigma‐Aldrich). Primary antibodies and anti‐rabbit secondary antibodies (7074) were purchased from Cell Signalling Technologies [pAKT S473 (9271), pCREB S133 (9198), PKC motif (6967), PKA motif (9624), pS6 S235/6 (4858), pRB S807/11 (8516), CaMKII S286 (12716), pMAPKAPK2 T222 (3316), p‐cJun S73 (3270), p‐cRAF S338 (9427), pACC S79 (11818), pHSP27 S82 (9709), AKT (4691) and CREB (9197)]. Antibodies against β‐Actin (AC‐15), GAPDH (MAB374) and EDNRB (21,196) were purchased from Sigma‐Aldrich, Millipore and Santa Cruz, respectively. Anti‐mouse and anti‐goat secondary antibodies were purchased from Jackson ImmunoResearch.
Schäfer A., Gjerga E., Welford R.W., Renz I., Lehembre F., Groenen P.M., Saez‐Rodriguez J., Aebersold R, & Gstaiger M. (2019). Elucidating essential kinases of endothelin signalling by logic modelling of phosphoproteomics data. Molecular Systems Biology, 15(8), e8828.
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2

Comprehensive Signaling Pathway Analysis

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Antibodies recognizing pan-AKT, p-AKT (S473), GSK3β, p-GSK3β (S9), TSC2, p-TSC2 (T1462), S6K, p-S6K (T389), S6, p-S6 (S235/6), PRAS40, ULK1, Rag A, Rag B, Rag C, AMPK, cleaved PARP, and Mcl-1 were from Cell Signaling Technology. β-tubulin antibody was from Sigma-Aldrich. XPB (TFIIH p89 S-19), p53, c-Myc, and GFP antibodies were from Santa Cruz. puromycin antibody was from KeraFAST. REDD1/DDIT4 antibodies were from Novus and Bethyl. C-FLIP antibody was from Enzo Life Sciences. All antibodies were used at final concentrations of 1:1000 – 1:2000 except for cleaved PARP, c-Myc, p-TSC2, ULK1, and XPB (1:500), AMPK, P70S6K, and TSC2 (1:750), and β-tubulin, S6, and p-S6 (1:4000). DMSO, cycloheximide, puromycin, rapamycin, bafilomycin A1, and MG-132 were from Sigma-Aldrich. Hygromycin B was from Corning. Hexadecylsulfonylfluoride (HDSF) was from Santa Cruz. TW-37 was from Selleckchem. Torin1 was a kind gift from David Sabatini and Nathanael Gray. Smac mimetic was a kind gift from Xiaodong Wang.
Potts M.B., McMillan E.A., Rosales T.I., Kim H.S., Ou Y.H., Toombs J.E., Brekken R.A., Minden M.D., MacMillan J.B, & White M.A. (2015). Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nature chemical biology, 11(6), 401-408.
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3

Comprehensive Signaling Pathway Analysis

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Antibodies recognizing pan-AKT, p-AKT (S473), GSK3β, p-GSK3β (S9), TSC2, p-TSC2 (T1462), S6K, p-S6K (T389), S6, p-S6 (S235/6), PRAS40, ULK1, Rag A, Rag B, Rag C, AMPK, cleaved PARP, and Mcl-1 were from Cell Signaling Technology. β-tubulin antibody was from Sigma-Aldrich. XPB (TFIIH p89 S-19), p53, c-Myc, and GFP antibodies were from Santa Cruz. puromycin antibody was from KeraFAST. REDD1/DDIT4 antibodies were from Novus and Bethyl. C-FLIP antibody was from Enzo Life Sciences. All antibodies were used at final concentrations of 1:1000 – 1:2000 except for cleaved PARP, c-Myc, p-TSC2, ULK1, and XPB (1:500), AMPK, P70S6K, and TSC2 (1:750), and β-tubulin, S6, and p-S6 (1:4000). DMSO, cycloheximide, puromycin, rapamycin, bafilomycin A1, and MG-132 were from Sigma-Aldrich. Hygromycin B was from Corning. Hexadecylsulfonylfluoride (HDSF) was from Santa Cruz. TW-37 was from Selleckchem. Torin1 was a kind gift from David Sabatini and Nathanael Gray. Smac mimetic was a kind gift from Xiaodong Wang.
Potts M.B., McMillan E.A., Rosales T.I., Kim H.S., Ou Y.H., Toombs J.E., Brekken R.A., Minden M.D., MacMillan J.B, & White M.A. (2015). Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nature chemical biology, 11(6), 401-408.
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