A transfected Chinese hamster ovary (CHO), Hela, a murine interleukin-3 dependent pro-B (Ba/F3), PC12, and rat basophil leukemia cell lines were obtained from Eurofins Scientific (Eurofins-Cerep, Le Bois I’Eveque, France). Buffers—Dulbecco’s modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and Hank’s balanced salt solution (HBSS) buffer—were purchased from Invitrogen (Carlsbad, CA, USA). The reference agonists: 5′-N-ethylcarboxamidoadenosine (NECA), epinephrine bitartrate, epinephrine, DPDPE, CP 55940, linoleic acid, GLP-1(7–37, arginine vasopressin (AVP), and serotonin, and antagonists: ZM 241385, RX-821002, rauwolscine, naltriben mesylate, AM 281, exendin-3(9–39), [d(CH2)51, Tyr (Me)2]-AVP, (S)-WAY-100635, and GR55562) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents purchased from Merck and Fluka were of the highest available grade unless otherwise stated.
Hank s balanced salt solution hbss buffer
Manufactured by Thermo Fisher Scientific
Sourced in United States
Hank's Balanced Salt Solution (HBSS) is a widely used buffer solution for cell culture applications. It is a balanced salt solution that maintains the physiological pH, osmolarity, and ionic composition necessary for the survival and growth of cells in vitro. HBSS is a versatile buffer that can be used for a variety of cell-based experiments and assays.
Automatically generated - may contain errors
Lab products found in correlation
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes buffer, by Thermo Fisher Scientific (6 mentions)
S way 100635, by Merck Group (5 mentions)
Dulbecco s modified eagle medium dmem buffer, by Thermo Fisher Scientific (4 mentions)
Clozapine, by Merck Group (3 mentions)
Butaclamol, by Merck Group (3 mentions)
B ba f3, by Eurofins (2 mentions)
L 733 060, by Merck Group (2 mentions)
Sch23390, by Merck Group (2 mentions)
Epinephrine bitartrate, by Merck Group (1 mentions)
Rauwolscine, by Merck Group (1 mentions)
Zm241385, by Merck Group (1 mentions)
5 n ethylcarboxamidoadenosine neca, by Merck Group (1 mentions)
Cp55940, by Merck Group (1 mentions)
Glp 1 7 37, by Merck Group (1 mentions)
Rx 821002, by Merck Group (1 mentions)
Am281, by Merck Group (1 mentions)
Arginine vasopressin avp, by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
Dpdpe, by Merck Group (1 mentions)
Epinephrine, by Merck Group (1 mentions)
7 protocols using hank s balanced salt solution hbss buffer
1
Cell Lines and Reagents for Bioassays
2
Murine Pro-B and Transfected CHO Cell Lines
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A murine interleukin-3-dependent
pro-B (Ba/F3) and a transfected Chinese hamster ovary (CHO) cell lines
were obtained from Eurofins Scientific (Le Bois I’Eveque, France).
Dulbecco’s modified Eagle’s medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) buffer, and Hank’s balanced salt solution (HBSS)
buffer were purchased from Invitrogen (Carlsbad, CA). The reference
agonists (dopamine, serotonin, and arginine vasopressin) and antagonists
(SCH-23390, (+)-butaclamol, clozapine, (S)-WAY-100635, and [d(CH2)5,1 (link) Tyr(Me)2]-AVP) were obtained from Sigma-Aldrich (St. Louis, MO). All of the
drugs, chemicals, and reagents were of the highest grade available.
pro-B (Ba/F3) and a transfected Chinese hamster ovary (CHO) cell lines
were obtained from Eurofins Scientific (Le Bois I’Eveque, France).
Dulbecco’s modified Eagle’s medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) buffer, and Hank’s balanced salt solution (HBSS)
buffer were purchased from Invitrogen (Carlsbad, CA). The reference
agonists (dopamine, serotonin, and arginine vasopressin) and antagonists
(SCH-23390, (+)-butaclamol, clozapine, (S)-WAY-100635, and [d(CH2)5,1 (link) Tyr(Me)2]-AVP) were obtained from Sigma-Aldrich (St. Louis, MO). All of the
drugs, chemicals, and reagents were of the highest grade available.
3
Transfected Cell Lines and Reference Agonists/Antagonists
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The transfected Chinese hamster ovary (CHO) cells, rat basophil leukemia (RBL) cells, U373 cells and BA/F3 cells were obtained from Eurofins Scientific (Le Bois I’Eveque, France). Various buffers namely Hank’s balanced salt solution (HBSS) buffer, Dulbecco’s modified Eagle medium (DMEM) buffer and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer were obtained from Invitrogen (Carlsbad, CA, USA). The reference agonists and/or antagonists atropine sulphate salt, acetylcholine chloride, [Sar9, Met(O2)11]-Substance P, [Arg8]-vasopressin (AVP), (+)butacamol, clozapine, dopamine, [d(CH2)5 1, Tyr(Me)2]-AVP, serotonin, SCH 2330, L-733,060, (S)-WAY-100635 and 3-isobutyl-1-methylxanthine (IBMX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All the other chemicals and reagents used were purchased from E. Merck, Fluka (Rupert-Mayer-Str., Munich, Germany), and Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated, and were of highest grade available.
4
Characterization of Cell Lines for Drug Screening
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Human endogenous (U373MG cells), murine interleukin-3 dependent pro-B (Ba/F3) and a transfected Chinese hamster ovary (CHO) cell lines were obtained from Eurofins Scientific (Le Bois I’Eveque, France). Buffers—Dulbecco’s modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer and Hank’s balanced salt solution (HBSS) buffer—were purchased from Invitrogen (Carlsbad, CA, USA). The reference agonists dopamine, [Sar9, Met(O2)11]-SP, serotonin and arginine vasopressin, and antagonists (+) butaclamol, L 733060, (S)-WAY-100635, and [d(CH2)51,Tyr(Me)2]-AVP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents were purchased from Merck and Fluka, unless otherwise stated and were of highest available grade.
5
Monoamine Oxidase Enzyme Assay Protocol
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We purchased Human recombinant
monoamine oxidase (hMAO) isozymes, deprenyl, clorgyline-HCl, safinamide
mesylate salt, emodin, and dimethyl sulfoxide (DMSO) from Sigma-Aldrich
Co. (St Louis, MO, USA). The MAO-GloTM assay kit was purchased from
Promega (Promega Corporation, Madison, WI, USA). A transfected Chinese
hamster ovary (CHO) cell line was purchased from Eurofins Scientific
(Le Bois I’Eveque, France). Buffers—Dulbecco’s
modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) buffer, and Hank’s balanced salt solution (HBSS)
buffer from Invitrogen (Carlsbad, CA, USA). The reference agonist
arginine vasopressin and antagonist were purchased from Sigma-Aldrich
(St. Louis, MO, USA). All the drugs, chemicals, and reagents were
of the highest grade available and were purchased from Merck (Darmstadt,
Germany) and Fluka (Buchs, Switzerland), unless otherwise stated.
monoamine oxidase (hMAO) isozymes, deprenyl, clorgyline-HCl, safinamide
mesylate salt, emodin, and dimethyl sulfoxide (DMSO) from Sigma-Aldrich
Co. (St Louis, MO, USA). The MAO-GloTM assay kit was purchased from
Promega (Promega Corporation, Madison, WI, USA). A transfected Chinese
hamster ovary (CHO) cell line was purchased from Eurofins Scientific
(Le Bois I’Eveque, France). Buffers—Dulbecco’s
modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) buffer, and Hank’s balanced salt solution (HBSS)
buffer from Invitrogen (Carlsbad, CA, USA). The reference agonist
arginine vasopressin and antagonist were purchased from Sigma-Aldrich
(St. Louis, MO, USA). All the drugs, chemicals, and reagents were
of the highest grade available and were purchased from Merck (Darmstadt,
Germany) and Fluka (Buchs, Switzerland), unless otherwise stated.
6
Isolation and Characterization of Novel Lipid Precursors
7
Recombinant hMAO Inhibition and GPCR Functional Assays
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For the recombinant hMAO inhibition assay, a MAO-A/B assay kit (Promega Cooperation, Madison, WI, USA), recombinant hMAO isoenzymes, and the standards l -deprenyl⋅HCl (Sigma Aldrich, St. Louis, MO, USA) were used. For the GPCR functional assay, reference drugs of DA, serotonin, AVP, clozapine, butaclamol, SCH23390, (S)-WAY-100635 and [d(CH2)51, Tyr(Me)2]-AVP as well as the test compound, ILQ, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Transfected CHO and human embryonic kidney (HEK-293) cell lines were generated by Eurofins Discovery (Le Bois I’Eveque, France). The cell culture media of Roswell Park Memorial Institute (RPMI-1641), Dulbecco's modified Eagle medium (DMEM) buffer, Hank’s balanced salt solution (HBSS) buffer, and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer were procured from ThermoFisher Scientific (USA).
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