Fitc labeled goat anti mouse antibody

Manufactured by Abcam

FITC-labeled goat anti-mouse antibody is a secondary antibody labeled with the fluorescent dye fluorescein isothiocyanate (FITC). It is designed to bind to mouse primary antibodies, allowing for their detection in various immunoassays and imaging applications.

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Lab products found in correlation

Prolong diamond antifade with dapi, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Tcs sp5 confocal laser microscope, by Leica (1 mentions) Anti opn, by Abcam (1 mentions) X40 magnification, by Olympus (1 mentions)

Close spelling variants of this product

Goat anti-mouse FITC (13 citations) Mouse anti (2 citations) FITC-labeled goat anti-mouse IgG secondary antibody (2 citations)

3 protocols using fitc labeled goat anti mouse antibody

Evaluating Bronchial and Liver Organoids

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Example 3

Determining Morphology of Bronchial Organotypic Cultures and Liver Spheroids

Morphology of bronchial organotypic cultures is evaluated following fixation and paraffin embedding, sectioning and staining with hematoxylin and eosin (H&E) and Alcian blue as previously described in Toxicol Sci. 2015 September; 147(1):207-21.

Liver spheroid morphology is assessed following immunostaining. In brief, liver spheroids are fixed in 4% fresh paraformaldehyde overnight. Following blocking in 1% Triton X-100/0.2% fish skin gelatin (FSG), spheroids are stained with mouse anti-cytokeratin 19 (1/500, Abcam, Cambridge, UK) diluted in PBS with 0.1% FSG for 24 hours. The primary antibody is visualized using a FITC-labeled goat anti-mouse antibody (1/500, Abcam). Spheroids are then mounted using ProLong Diamond antifade with DAPI (Thermo Fisher) and evaluated by high-content imaging on the Cellinsight™ CX7 platform (Thermo Fisher).

US11041145B2. Cell culture (2021-06-22). PHILIP MORRIS PRODUCTS S.A. [CH]. Inventors: David Bovard [CH], Karsta Luettich [CH].

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Cell-Cell Fusion Assay with TMPRSS2 and MSP

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MDCK cells were seeded in 6-well plates, and 1 μg of pCAGGS-TMPRSS2-FLAG, pCAGGS-MSPL-FLAG, and pCAGGS was cotransfected with 1 μg of pCAGGS-Re-5HA, pCAGGS-Re-6HA, and pCAGGS-Re-9HA, respectively, using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA). 48 h after transfection, cells were treated with acidic PBS (pH 5.0) at room temperature for 5 min and replenished with DMEM containing 5% FBS and cultured at 37°C for 1 h. When fusion was obvious, cells were fixed with 3% paraformaldehyde and stained with mouse anti-FLAG antibody and chicken anti-Re-5, anti-Re-6, or anti-Re-9 as primary antibody with FITC-labeled goat anti-mouse antibody (Abcam, Cambridge, CA) and TRITC-labeled rabbit anti-chicken antibody (Abcam, Cambridge, CA) for 30 min. Cells were washed and stained with DAPI and images were acquired with a Leica TCS SP5 confocal laser microscope (Leica Microsystems, Wetzlar, Germany).

Wen Z., Wu C., Chen W., Zeng X., Shi J., Ge J., Chen H, & Bu Z. (2015). Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin. Biotechnology Research International, 2015, 402628.

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Immunofluorescence Staining of RASMCs

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RASMCs were fixed in 4% paraformaldehyde (cat. no. A0000700; Shanghai Richjoint Chemical Reagent Co., Ltd.) at room temperature for 30 min and incubated with 0.1% Triton at room temperature for 15 min. After blocking with 5% FBS for 30 min at 37˚C, the cells were incubated overnight with anti-α-SMA (1:500; cat. no. ab7817; Abcam) and anti-OPN (1:500; cat. no. ab8448; Abcam) primary antibodies at 4˚C. Subsequently, cells were incubated with FITC-labeled goat anti-mouse Antibody (1:200; cat. no. 31635; Thermo Fisher Scientific, Inc.) and Cy3-labeled goat anti-rabbit antibody (1:100; cat. no. BA1032; AMSBIO LLC.) for 45 min at 37˚C. Then, 0.5 ml Hoechst 33258 staining solution (5 µg/ml; cat. no. C1017; Beyotime Institute of Biotechnology) was added onto the glass slide loading cells sample for 5 min at room temperature. Finally, the samples were observed under a fluorescence microscope with x40 magnification (Olympus Corporation). Image analysis was performed using ImageJ software (version 1.8.0; National Institutes of Health).

Wang L., Zhao L.P., Chen Y.Q., Chang X.S., Xiong H., Zhang D.M., Xu W.T, & Chen J.C. (2021). Adropin inhibits the phenotypic modulation and proliferation of vascular smooth muscle cells during neointimal hyperplasia by activating the AMPK/ACC signaling pathway. Experimental and Therapeutic Medicine, 21(6), 560.

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