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Nanozoomer ndp view2

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The NanoZoomer NDP.view2 is a digital pathology slide viewing software developed by Hamamatsu Photonics. It provides functionality for viewing and analyzing digital slides scanned using Hamamatsu's NanoZoomer digital slide scanners.

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Nanozoomer, by Hamamatsu Photonics (1 mentions)

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NanoZoomer (461 citations) NDP.view2 software (285 citations) Nanozoomer NDP (9 citations) View2 software (8 citations) NanoZoomer Digital Pathology NDP.view2 (2 citations)

3 protocols using nanozoomer ndp view2

1

Quantifying Cardiomyocyte Apoptosis Post-Infarction

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To analyze TUNEL staining, ten FOVs per animal from the infarct were exported at ×40 using NanoZoomer NDP.view2 software (Hamamatsu). For each FOV, we manually counted troponin+ DAPI+ TUNEL+ cardiomyocytes using ImageJ 1.8.0_172. Infarct size was quantified using ImageJ 1.8.0_172.
Grune J., Lewis A.J., Yamazoe M., Hulsmans M., Rohde D., Xiao L., Zhang S., Ott C., Calcagno D.M., Zhou Y., Timm K., Shanmuganathan M., Pulous F.E., Schloss M.J., Foy B.H., Capen D., Vinegoni C., Wojtkiewicz G.R., Iwamoto Y., Grune T., Brown D., Higgins J., Ferreira V.M., Herring N., Channon K.M., Neubauer S., Sosnovik D.E., Milan D.J., Swirski F.K., King K.R., Aguirre A.D., Ellinor P.T, & Nahrendorf M. (2022). Neutrophils incite and macrophages avert electrical storm after myocardial infarction. Nature cardiovascular research, 1(7), 649-664.
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2

Quantifying Cardiomyocyte Apoptosis Post-Infarction

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To analyze TUNEL staining, ten FOVs per animal from the infarct were exported at ×40 using NanoZoomer NDP.view2 software (Hamamatsu). For each FOV, we manually counted troponin+ DAPI+ TUNEL+ cardiomyocytes using ImageJ 1.8.0_172. Infarct size was quantified using ImageJ 1.8.0_172.
Grune J., Lewis A.J., Yamazoe M., Hulsmans M., Rohde D., Xiao L., Zhang S., Ott C., Calcagno D.M., Zhou Y., Timm K., Shanmuganathan M., Pulous F.E., Schloss M.J., Foy B.H., Capen D., Vinegoni C., Wojtkiewicz G.R., Iwamoto Y., Grune T., Brown D., Higgins J., Ferreira V.M., Herring N., Channon K.M., Neubauer S., Sosnovik D.E., Milan D.J., Swirski F.K., King K.R., Aguirre A.D., Ellinor P.T, & Nahrendorf M. (2022). Neutrophils incite and macrophages avert electrical storm after myocardial infarction. Nature cardiovascular research, 1(7), 649-664.
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3

Adipocyte Morphometry in Murine WAT

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Mice were anesthetized with isoflurane and perfused with PBS followed by 4% PFA. The perigonadal WAT were fixed with 4% PFA overnight and placed into 70% ethanol. Paraffin sections were prepared by Tissue Tech VIRTM 5 Junior (VIP-5-Jr-10; Sakura Fine Chemical), and HE-staining was conducted by Multiple Slide Stainer (DRS 2000-B; Sakura Fine Chemical). Slide images were scanned by NanoZoomer (Hamamatsu Photonics). The sections were viewed at 20x magnification and randomly selected five areas each section using NanoZoomer NDP.view2 (Hamamatsu Photonics). The size of adipocytes was measured by ImageJ with Adipocyte_Tools.ijm. Genotypes and conditions were blinded during the experimental procedures.
Tsuji S., Brace C.S., Yao R., Tanie Y., Tada H., Rensing N., Mizuno S., Almunia J., Kong Y., Nakamura K., Furukawa T., Ogiso N., Toyokuni S., Takahashi S., Wong M., Imai S.I, & Satoh A. (2023). Sleep–wake patterns are altered with age, Prdm13 signaling in the DMH, and diet restriction in mice. Life Science Alliance, 6(6), e202301992.
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