HCEC cultures on eight-chamber slides were fixed with methanol at –20°C for 5 minutes. Indirect immunofluorescent staining was performed using our previous methods.53 (link) Primary rabbit polyclonal antibody against human LC3B (Novus Biologicals) was used. Alexa Fluor 488-conjugated AffiniPure Goat anti-Rabbit IgG (H+L) secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) was applied, and 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, Carlsbad, CA, USA) was used for nuclear counterstaining. Secondary antibody was applied alone as a negative control and compared to isotype goat IgG. The staining was photographed with a Nikon A1 Confocal Laser Microscope System (Nikon Instruments, Melville, NY, USA) and processed with Image J software (National Institutes of Health, Bethesda, MD, USA).
Alexa fluor 488 conjugated affinipure goat anti rabbit igg h l secondary antibody
Manufactured by Jackson ImmunoResearch
Sourced in United States
Alexa Fluor 488-conjugated AffiniPure Goat anti-Rabbit IgG (H+L) secondary antibody is a fluorescently labeled secondary antibody used to detect and visualize rabbit primary antibodies in various immunochemical applications. It is produced in goats and affinity-purified to ensure specificity. The Alexa Fluor 488 dye provides a green fluorescent signal.
Automatically generated - may contain errors
2 protocols using alexa fluor 488 conjugated affinipure goat anti rabbit igg h l secondary antibody
1
Indirect Immunofluorescent Staining of LC3B
2
Immunofluorescent Staining of LC3B in Primary HCECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary HCECs, cultured on 8-chamber slides, were fixed with methanol at –20 °C for 5 min. Indirect immunofluorescent staining was conducted as our previously described [49 (link)]. Primary rabbit polyclonal antibody against human LC3B (Novus Biologicals, Littleton, CO, USA) was used. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) was applied, and 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, Eugene, OR, USA) was used for nuclear counterstaining. As a negative control, secondary antibody was applied alone and compared to isotype goat IgG. The staining was then photographed with Nikon A1 Confocal Laser Microscope System (Nikon Instruments, Melville, NY, USA) and processed with Image J software (National Institutes of Health, Bethesda, MD, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!