Prazosin p7791

The cholinergic, noradrenergic and glutamatergic Mecamylamine M9020, Yohimbine Y3125, Propranolol P0884, Prazosin P7791, and atropine were purchased from Sigma. Drugs were dissolved in warm cortex buffer and filtered and then injected into cortex using a glass micropipette using a picospritzer. During the initial surgery and AAV injection, a hole was drilled through each coverslip which was ultimately used to form the cranial window. This hole was plugged using kwik-sil and covered with acrylic to prevent desiccation and infection. During imaging, 2 weeks after AAV injection, the kwik-sil was removed, GCaMP6-expressing neurons were imaged for 15 minutes, and then the mouse was removed from the microscope and placed under a dissection microscope. The glass micropipette containing the filtered drugs was then inserted into the cortex via the previously-drilled hole. This permitted the maintenance of the cranial window intact. The mouse was then placed back under the 2-photon microscope, the same neurons were again found, and their activities imaged for another 15 minutes.