Human rec ngal

Manufactured by R&D Systems

Human rec-NGAL is a recombinant human protein. It is used for research purposes in cell culture and in vitro studies.

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Lab products found in correlation

Mouse recombinant rec lcn2, by Cell Signaling Technology (2 mentions) Lactate dehydrogenase, by Randox (1 mentions) Sybr green mix, by Quanta Biosciences (1 mentions) Qscript cdna synthesis kit, by Quanta Biosciences (1 mentions) Creatinine, by Randox (1 mentions) Ferric chloride, by Merck Group (1 mentions) Guaiacol 2 methoxyphenol, by Thermo Fisher Scientific (1 mentions) Bovine milk lpo, by Worthington (1 mentions) Human mpo, by R&D Systems (1 mentions) Chrome azurol s, by Thermo Fisher Scientific (1 mentions) Pipes, by Merck Group (1 mentions) Epigallocatechin gallate (egcg), by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

2 protocols using human rec ngal

Mouse Inflammation and Biomarker ELISA Protocol

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Duoset mouse Lcn2 and CXCL1 (KC) ELISA kit, and anti-mouse Lcn2 antibody were obtained from R&D Systems. Aspartate transaminase (AST), alanine transaminase (ALT) triglycerides (TG), cholesterol (CHOL), lactate dehydrogenase (LDH) and creatinine kits were procured from Randox Laboratories. Glucometer and glucose test strips were obtained from Nova Max. Iron atomic absorption (AA) standard was purchased from RICCA Chemical Company. Ferrous sulfate was obtained from Alfa Aesar. SYBR® Green mix and qScript cDNA synthesis kit was procured from Quanta Biosciences. Bleomycin sulfate was procured from Biotang. Reagent grade Dextran Sulfate Sodium salt (DSS, reagent grade, M.W. 36–50 kDa, Ref = 160110) was purchased from MP Biomedicals. Mouse recombinant (rec)-Lcn2 was obtained from Cell Signaling and human rec-NGAL was acquired from R&D Systems; both of which are free from endotoxin, siderophore, and iron. All other fine chemicals used in present study were reagent grade and procured from Sigma.

Xiao X., Yeoh B.S., Saha P., Olvera R.A., Singh V, & Vijay-Kumar M. (2016). Lipocalin 2 Alleviates Iron Toxicity by Facilitating Hypoferremia of Inflammation and Limiting Catalytic Iron Generation. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 29(3), 451-465.

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Recombinant Protein Procurement for Biochemical Assays

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Mouse recombinant (rec)-Lcn2 was obtained from Cell Signalling and human rec-NGAL was acquired from R&D Systems; both of which are free from endotoxin, siderophore, and iron. Human MPO was procured from R&D Systems (Minneapolis, MN). EGCG, PMA, ferric chloride, PIPES, agar, and H₂O₂ were procured from Sigma-Aldrich (St. Louis, MO). Bovine milk LPO was purchased from Worthington Biochemical Corp (Lakewood, NJ). Chrome Azurol S was purchased from Acros Organics. Guaiacol (2-methoxyphenol) was obtained from Alfa Aesar (Ward Hill, MA).

Saha P., Yeoh B.S., Singh R., Chandrasekar B., Vemula P.K., Haribabu B., Vijay-Kumar M, & Jala V.R. (2016). Gut Microbiota Conversion of Dietary Ellagic Acid into Bioactive Phytoceutical Urolithin A Inhibits Heme Peroxidases. PLoS ONE, 11(6), e0156811.

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