Rat anti cd8

Manufactured by Abcam

Sourced in United States

Rat anti-CD8 is an antibody that binds to the CD8 protein found on the surface of certain T cells. CD8 is a co-receptor that assists in the activation of cytotoxic T cells. The antibody can be used for the identification and analysis of CD8-positive T cells in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Rat anti f4 80, by Abcam (1 mentions) Rat anti gr 1, by Thermo Fisher Scientific (1 mentions) Rabbit anti phospho p44 42 mapk thr202 tyr204, by Cell Signaling Technology (1 mentions) Rat anti cd68, by Abcam (1 mentions) Rat anti cd4, by Thermo Fisher Scientific (1 mentions) Rat anti b220, by Thermo Fisher Scientific (1 mentions) Rat anti cd4 gk1.5, by Abcam (1 mentions) Axioplan 2, by Zeiss (1 mentions) Alexa 488 streptavidin, by Jackson ImmunoResearch (1 mentions) Alexa 594 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Alexa 488, by Jackson ImmunoResearch (1 mentions)

2 protocols using rat anti cd8

Immunohistochemical Analysis of EPRAP and Associated Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit polyclonal antibody against mouse EPRAP was raised by immunization with keyhole limpet hemocyanin–conjugated synthetic peptides corresponding to amino-acid residues 244–260, 330–346, and 629–645 of murine EPRAP. For immunohistochemistry of mouse samples, paraffin-embedded sections were routinely stained with one of the following antibodies: rat anti-F4/80 (Abcam, Cambridge, MA, USA), rat anti–Gr-1 (eBioscience, San Diego, CA, USA), rat anti-B220 (eBioscience), rat anti-CD4 (eBioscience), rat anti-CD8 (Abcam), hamster anti-CD11c (eBioscience), mouse anti-NCAM (Abcam), rabbit anti–phospho-NF-κB p105 (Ser933) (Cell Signaling, Boston, MA, USA), rabbit anti–phospho-MEK1/2 (Ser221) (Cell Signaling), or rabbit anti–phospho-p44/42 MAPK (Thr202/Tyr204) (Cell Signaling), or rabbit anti-EPRAP.
For immunohistochemistry of human samples, paraffin-embedded sections were stained with rat anti-CD68 (Abcam), goat anti-FEM1A (Abcam), and rabbit anti–phospho-NF-κB p105 (Ser933), rabbit anti–phospho-MEK1/2 (Ser221), or rabbit anti–phospho-p44/42 MAPK (Thr202/Tyr204) antibodies. Staining with non-immune rat or rabbit IgG served as a negative control for each experiment.

Nakatsuji M., Minami M., Seno H., Yasui M., Komekado H., Higuchi S., Fujikawa R., Nakanishi Y., Fukuda A., Kawada K., Sakai Y., Kita T., Libby P., Ikeuchi H., Yokode M, & Chiba T. (2015). EP4 Receptor–Associated Protein in Macrophages Ameliorates Colitis and Colitis-Associated Tumorigenesis. PLoS Genetics, 11(10), e1005542.

+ Open protocol

+ Expand

Immunostaining of Thymic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unfixed thymi were embedded in OCT compound (Sakura, Chuo-ku, Tokyo, Japan), cut, fixed in acetone and stained as previously described.^{49 (link)} The primary Abs used were rabbit anti-FADD (EP887Y), rabbit anti-Notch1 (EP1238Y), rabbit anti-Jagged1 (EPR4290), rabbit anti-Jagged2 (EPR3646) (Epitomics), rabbit anti-NKAP (Sigma), rat anti-CD4 (GK1.5), rat anti-CD8 (YTS169.4), rabbit anti-DL1 (30B11.1(14)), rabbit anti-DL4 (Abcam, Cambridge, MA, USA), rat anti-CD44 (IM7), and biotinylated rat anti-CD25(PC61) (BD Biosciences). Secondary reagents used were Alexa 488-streptavidin, Alexa 488 and Alexa 594-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, USA). Sections were first blocked for 1 h using 4% goat serum, then labeled with primary Abs followed by the secondary Abs. Nuclear DNA was stained using 4′, 6-diamidino-2-phenylindole (DAPI). Images were then visualized by microscopy (Carl Zeiss Axioplan 2, Göttingen, Germany).

Zhang X., Dong X., Wang H., Li J., Yang B., Zhang J, & Hua Z.C. (2014). FADD regulates thymocyte development at the β-selection checkpoint by modulating Notch signaling. Cell Death & Disease, 5(6), e1273-.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!