All PCR reactions were carried out on a C1000 thermal cycler (BioRad). DNA sequencing was performed by Elim BioPharm Inc. Preparative scale reverse-phase analytical and semipreparative HPLC was performed using a Dionex UHPLC with a photo-diode array UV/Vis detector (Thermo Fisher Scientific). HRMS analysis was conducted using a Q Exactive Benchtop Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with a Dionex RSLC (Thermo Fisher Scientific). Conventional low-resolution LC-MS analysis was conducted with a Shimadzu LCMS-2020 system. The NMR spectrum was recorded on a Bruker AV600 (600 MHz) spectrometer or a Bruker Avance III 700 MHz spectrometer equipped with a 1H/13C/15N triple-resonance inverse probe (1.7 mm microprobe).
Dionex rslc
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The Dionex RSLC is a high-performance liquid chromatography (HPLC) system designed for reliable, sensitive, and efficient analysis of a wide range of samples. It features advanced technology and robust design to deliver consistent and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
Avance 3 700 mhz spectrometer, by Bruker (2 mentions)
Lcms 2020 system, by Shimadzu (2 mentions)
Dionex uhplc, by Thermo Fisher Scientific (2 mentions)
Uv vis photo diode array detector, by Thermo Fisher Scientific (2 mentions)
Q exactive benchtop quadrupole orbitrap mass spectrometer, by Thermo Fisher Scientific (2 mentions)
Av600 600 mhz spectrometer, by Bruker (2 mentions)
1h 13c 15n triple resonance inverse probe 1.7 mm microprobe, by Bruker (2 mentions)
C1000 thermal cycler, by Bio-Rad (2 mentions)
Ultimate 3000, by Thermo Fisher Scientific (1 mentions)
Agilent 1100 series, by Agilent Technologies (1 mentions)
3 protocols using dionex rslc
1
Analytical Characterization of Compounds
2
Analytical Characterization of Compounds
3
NMR and Mass Spectrometric Analysis
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NMR spectra were obtained on a Bruker Ascend 500 MHz spectrometer equipped with a cryoprobe (Bruker Biospin GmbH, Waldbronn, Germany), in the solvents indicated and referenced to residual 1 H signals in deuterated solvents. ESI-MS were acquired using an Agilent 1100 Series (Agilent Technologies, Waldbronn, Germany) separations module equipped with an Agilent 1100 Series LC/MSD mass detector in both positive and negative ion modes under the following conditions: Zorbax C 8 column, 150 × 4.6 mm, eluting with 95% H 2 O/MeCN to 5% H 2 O/MeCN (with isocratic 0.01% TFA) over 22 min at 0.4 ml min -1 and then held for 5 min. HRMS was carried out using an UltiMate 3000 rapid separation liquid chromatography system (Dionex RSLC, Thermo Fisher Scientific, Dreieich, Germany) coupled to an UHR-TOF mass spectrometer (Bruker Daltonik MaXis, Bruker Daltonik GmbH, Bremen, Germany) operating in the positive ESI mode.
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