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2 protocols using glycerin

1

Wound Healing Gel Formulation

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The chemical materials used in this study include SPRC (also named ZYZ‐802, Shanghai), carbomer 940 (Macklin, China), sodium carboxymethyl cellulose (Macklin), glycerin (Macklin), sodium hydroxide (Macklin), Tween‐20 (Sigma, USA), Tween‐80 (Macklin), TRIzol reagent (Carlsbad, CA, USA), and phosphate‐buffered saline (PBS) tablets (Gaithersburg, MD, USA). Recombinant bovine bFGF was purchased from Essex Bio‐Technology (Zhuhai, China). Hydrogen peroxide solution was obtained from ANNJET (Shandong, China). Alcohol, n‐butanol, and xylene were purchased from SINOPHARM (Shanghai, China). Masson Tricolor Staining Kit, HE Staining Kit, BioDewax and clear solution, tris‐ethylene diamine tetraacetic acid antigen retrieval solution (Tris‐EDTA), pH = 9.0), bovine serum albumin (BSA), horseradish peroxidase (HRP) conjugated goat anti‐rabbit IgG (H+L), and DAB chromogenic kit were purchased from Servicebio (Wuhan, China). Total RNA was extracted with RNA Extraction Kit was purchased from Solarbio (Beijing, China). The HiFiScript gDNA Removal cDNA Synthesis Kit was obtained from CWBIO (Jiangsu, China). The PerfectStar Green qPCR SuperMix (+Dye I/+Dye II) was purchased from TRANS (Beijing, China).
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2

Synthesis and Characterization of Sodium Alginate Hydrogel

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SA (white powder, Mw = 220 kDa, MWD
= 1.5 and M/G = 1.05 as we
used before30 (link)) was bought from the Gather
Great Ocean Algae Industry Group. Glutaraldehyde was obtained from
Tianjin Bodi Chemical Industry Co. Hydrochloric acid (HCl, 36–38%)
was obtained from Beijing Chemical Reagent Co. Sodium hydroxide (NaOH,
AR) was purchased from Sinopharm Chemical Reagent Co. The metal salts
needed for the experiment were purchased from Sinopharm Chemical Reagent
Co., and all reagents were of analytical grade. MTT was purchased
from China Center for Type Culture Collection (Wuhan, China). Glycerin
(99%) was purchased from Shanghai Macklin Biochemical Co. Secondary
deionized water was used throughout.
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