All general chemicals, unless noted, were from Thermo Fisher Scientific (Waltham, MA). Antibodies used for immunoblotting: anti-Cx43, Cx43NT1 made at the Fred Hutchinson Cancer Research Center Antibody Technology Facility (Seattle, WA); E-cadherin, #3195 Cell Signaling Technology (Danvers, MA); NDRG1, Vimentin, #13901 Cell Signaling Technology; Vinculin V4505 MillliporeSigma (Burlington, MA); Immunohistochemistry: Cx43 (6219, MilliporeSigma); Ki67 (12202 Cell Signaling Technology), Progesterone receptor (Lab Vision SP2, Thermo Fisher), Vimentin (Novus Biologicals, Centennial, CO); Immunofluorescence: anti-PCNA (NA03, MilliporeSigma), CK19 (TROMA-3 Developmental Studies Hybridoma Bank (Iowa City, IA)), smooth muscle actin (A5228 MiliporeSigma). Secondary antibodies from ThermoFisher: Rat IgG 647 (A21247); anti-rabbit IgG 594 (A32754); anti-mouse IgG2a 488 (A32723).
Anti mouse igg2a 488
Manufactured by Thermo Fisher Scientific
Anti-mouse IgG2a 488 is a fluorescently labeled secondary antibody that binds to mouse IgG2a antibodies. It is designed for use in immunoassays and other applications that require the detection of mouse IgG2a.
Automatically generated - may contain errors
2 protocols using anti mouse igg2a 488
1
Immunoblotting and Immunohistochemistry Protocol
2
Immunoblotting and Immunohistochemistry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All general chemicals, unless noted, were from Thermo Fisher Scientific (Waltham, MA). Antibodies used for immunoblotting: anti-Cx43, Cx43NT1 made at the Fred Hutchinson Cancer Research Center Antibody Technology Facility (Seattle, WA); E-cadherin, #3195 Cell Signaling Technology (Danvers, MA); NDRG1, Vimentin, #13901 Cell Signaling Technology; Vinculin V4505 MillliporeSigma (Burlington, MA); Immunohistochemistry: Cx43 (6219, MilliporeSigma); Ki67 (12202 Cell Signaling Technology), Progesterone receptor (Lab Vision SP2, Thermo Fisher), Vimentin (Novus Biologicals, Centennial, CO); Immunofluorescence: anti-PCNA (NA03, MilliporeSigma), CK19 (TROMA-3 Developmental Studies Hybridoma Bank (Iowa City, IA)), smooth muscle actin (A5228 MiliporeSigma). Secondary antibodies from ThermoFisher: Rat IgG 647 (A21247); anti-rabbit IgG 594 (A32754); anti-mouse IgG2a 488 (A32723).
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