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Microcal peq itc analysis software

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MicroCal PEQ-ITC analysis software is a tool used to analyze data from Isothermal Titration Calorimetry (ITC) experiments. It provides data analysis and visualization capabilities for researchers studying biomolecular interactions.

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Lab products found in correlation

Vp itc microcalorimeter, by Malvern Panalytical (1 mentions) Microcal itc200 instrument, by Malvern Panalytical (1 mentions) Peaq itc instrument, by Malvern Panalytical (1 mentions)

2 protocols using microcal peq itc analysis software

1

Isothermal Titration Calorimetry of DarB

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ITC experiments were carried out with a VP-ITC microcalorimeter (MicroCal Inc., Northampton, MA) to determine the affinity of DarB to c-di-AMP or RelNTD. In a typical setup, DarB (10 μM) was placed in the sample cell, and c-di-AMP (100 μM in the same buffer) was placed in the titration syringe. All experiments were carried out at 20°C with a stirring speed of 329 rpm. The parameters used for the titration series are given in Table S4. Data analysis was carried out using MicroCal PEQ-ITC analysis software (MalvernPanalytical).
Krüger L., Herzberg C., Wicke D., Scholz P., Schmitt K., Turdiev A., Lee V.T., Ischebeck T, & Stülke J. (2022). Sustained Control of Pyruvate Carboxylase by the Essential Second Messenger Cyclic di-AMP in Bacillus subtilis. mBio, 13(1), e03602-21.
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2

Isothermal Titration Calorimetry of Phosphopeptides

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ITC experiments were conducted using the Malvern MicroCal ITC200 instrument (monophosphorylated peptides) or Malvern PEAQ ITC instrument (di-phosphorylated peptide). The ITC conditions used are detailed below in Table S5. All experiments were conducted in buffer containing 25 mM Hepes pH 7.5, 100 mM NaCl, 10 mM MgCl2, and 1% v/v DMSO. Where two titrations were conducted, the data was merged using Concat32 software (Malvern Instruments Ltd) All data was analyzed using MicroCal PEQ-ITC Analysis Software (single site binding model) to obtain parameters (where applicable).
Ward J.A., Romartinez-Alonso B., Kay D.F., Bellamy-Carter J., Thurairajah B., Basran J., Kwon H., Leney A.C., Macip S., Roversi P., Muskett F.W, & Doveston R.G. (2024). Characterizing the protein–protein interaction between MDM2 and 14-3-3σ; proof of concept for small molecule stabilization. The Journal of Biological Chemistry, 300(2), 105651.
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