Duoset elisa for human il 29 il 28b ifnl 1 3 kit

Manufactured by R&D Systems

The DuoSet ELISA for Human IL-29/IL-28B (IFNL-1/3) kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-29 (IL-29) and interleukin-28B (IL-28B) in cell culture supernatants, serum, and plasma.

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β actin, by Merck Group (1 mentions) Rig 1, by Cell Signaling Technology (1 mentions)

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DuoSet ELISA kits (1131 citations) IL-29 (8 citations) Human IL-3 (7 citations) IL-28B (5 citations) Human IL-29 DuoSet ELISA (2 citations) Human IL‐29/IL‐28B ( (2 citations) IL-28b/IL-29 (2 citations) IL-3 DuoSet ELISA kit (2 citations) DuoSet ELISA kit for human (2 citations) Human ELISA DuoSet (2 citations)

2 protocols using duoset elisa for human il 29 il 28b ifnl 1 3 kit

Innate Immune Pathway Protein Detection

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Western blot was performed using primary antibodies against cGAS (Cell Signaling Technology (CST), 15102S, 1:1000), EZH2 (CST, 5246S, 1:1000), GAPDH (CST, 5174S, 1:1000), H3K27me3 (CST, 9733S, 1:1000), Histone H3 (CST, 14269S, 1:1000), ING3 (Sigma-Aldrich, HPA067575, 1:500), IRF3 (CST, 11904S, 1:1000), IRF7 (CST, 72073S, 1:1000), MAVS (CST, 3993S, 1:1000), MDA5 (CST, 5321S,1:1000), RIG-I (CST, 3743S, 1:1000), STING (CST, 13647S, 1:1000) and TBK1 (CST, 3504S, 1:1000). IFN-λ secretion was quantified by the DuoSet ELISA for Human IL-29/IL-28B (IFNL-1/3) kit (R&D Systems).

Song Y., Hou G., Diep J., Ooi Y.S., Akopyants N.S., Beverley S.M., Carette J.E., Greenberg H.B, & Ding S. (2021). Inhibitor of growth protein 3 epigenetically silences endogenous retroviral elements and prevents innate immune activation. Nucleic Acids Research, 49(22), 12706-12715.

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Immunoblotting and ELISA Analyses

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Western blot analyses employed antibodies against ERV-3 (1:1000, Everest), B-Actin (1:5000, Sigma), MDA5 (1:1000, Cell Signaling #5321), PARP (#9542, 1:1000; Cell Signaling Technology, Inc.), RIG-I (1:1000, Cell Signaling #4200), STING (1:1000, Abcam #ab82960), Syncytin-1 (1:350, Dr. Hervé Perron, Geneuro, Geneva Switzerland), and TLR3 (1:1000, Cell Signaling #6961). IFNB ELISA utilized the Verikine-HS™ Human Interferon Beta Serum ELISA kit (PBL Interferon Source) and IFNL ELISA the DuoSet ELISA for Human IL-29/IL28-B (IFNL 1/3) kit (R & D Systems).

Chiappinelli K.B., Strissel P.L., Desrichard A., Li H., Henke C., Akman B., Hein A., Rote N.S., Cope L.M., Snyder A., Makarov V., Buhu S., Slamon D.J., Wolchok J.D., Pardoll D.M., Beckmann M.W., Zahnow C.A., Mergoub T., Chan T.A., Baylin S.B, & Strick R. (2015). Inhibiting DNA methylation causes an interferon response in cancer via dsRNA including endogenous retroviruses. Cell, 162(5), 974-986.

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