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Sox9 ab5535 antibody

Manufactured by Merck Group
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The SOX9 AB5535 antibody is a laboratory reagent used to detect the presence and quantify the levels of the SOX9 protein in biological samples. SOX9 is a transcription factor that plays a crucial role in the regulation of various cellular processes, including chondrogenesis, sex determination, and neural crest development. This antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of the SOX9 protein in different cell types and tissues.

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Lab products found in correlation

Novex ecl chemi substrate, by Thermo Fisher Scientific (1 mentions) β actin ac 15, by Merck Group (1 mentions) Vimentin sc 6260, by Santa Cruz Biotechnology (1 mentions) Yap antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

AB5535 (128 citations) SOX9 antibody (6 citations)

2 protocols using sox9 ab5535 antibody

1

Western Blot Analysis of SOX Proteins

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Immunoblots were performed following standard procedures. For SOX2 detection, AB5603 antibody (Millipore, Billerica, MA, USA) was used, for SOX9 AB5535 antibody (Millipore), and for β-actin AC-15 (Sigma). HRP-linked (Horseradish Peroxidase) anti-rabbit or anti-mouse (SantaCruz Biotechnology, Dallas, TX, USA) secondary antibodies, both at a 1:2000 dilution, were used. Detection was performed by chemiluminescence using NOVEX ECL Chemi Substrate (ThermoFisher, Waltham, MA, USA).
Garros-Regulez L., Aldaz P., Arrizabalaga O., Moncho-Amor V., Carrasco-Garcia E., Manterola L., Moreno-Cugnon L., Barrena C., Villanua J., Ruiz I., Pollard S., Lovell-Badge R., Sampron N., Garcia I, & Matheu A. (2016). mTOR inhibition decreases SOX2-SOX9 mediated glioma stem cell activity and temozolomide resistance. Expert Opinion on Therapeutic Targets, 20(4), 393-405.
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2

Characterization of EMT Markers

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YAP antibody (#14074) was from Cell Signaling Technology. SOX9 (#AB5535) antibody was from Millipore. Antibodies against E-cadherin (sc-8426) and vimentin (sc-6260) were purchased from Santa Cruz Biotechnology. Anti-CD44 (EP44) and anti-vimentin (OTI5D7) antibodies were from ZSGB-BIO. siRNA against human YAP and SOX9, mimics and antisense antagomirs of miR-506-3p, and scramble control RNA oligos were purchased from GenePharma.
Wang L., Zhang Z., Yu X., Huang X., Liu Z., Chai Y., Yang L., Wang Q., Li M., Zhao J., Hou J, & Li F. (2018). Unbalanced YAP–SOX9 circuit drives stemness and malignant progression in esophageal squamous cell carcinoma. Oncogene, 38(12), 2042-2055.
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