Single-cell suspensions were isolated from the spleen of 2D2 TCR transgenic mice then cultured for 3 days with MOG35–55 (25 µg/mL), soluble anti-CD3 (0.5 μg/mL, Bio X Cell, BE0002), anti-CD28 (1 μg/mL, Bio X Cell, BE0015-5), IL-6 (20 ng/mL, R&D, 406-ML-025), TGF-β (2 ng/mL, R&D, 7666-MB-005), IL-1β (10 ng/mL, R&D, 401-ML-010), anti-IFN-γ (10 μg/mL, Bio X Cell, BE0054), and anti-IL-4 (10 μg/mL, Bio X Cell, BE0045) to induce differentiation into Th17 cells. Cells were analyzed by flow cytometry.
Be0045
Manufactured by BioXCell
BE0045 is a compact and versatile laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor capable of accommodating various sample tubes and microplates. The centrifuge operates at a maximum speed of 6,000 rpm and can generate a maximum relative centrifugal force (RCF) of 3,000 x g. The unit is equipped with a digital display for speed and time settings, as well as safety features to ensure secure operation.
Automatically generated - may contain errors
Lab products found in correlation
Anti il 4, by BioXCell (2 mentions)
Soluble anti cd3, by BioXCell (1 mentions)
Anti ifn γ, by BioXCell (1 mentions)
Anti cd28, by BioXCell (1 mentions)
Be0002, by BioXCell (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Tgf β, by R&D Systems (1 mentions)
Be0054, by BioXCell (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Sodium pyruvate, by Corning (1 mentions)
L glutamine, by Avantor (1 mentions)
Ril 12, by BioLegend (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
2 protocols using be0045
1
Th17 Cell Differentiation Protocol
2
Cytotoxic T Cell Isolation and Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Six-to-eight-week-old male and female mice were euthanized, and their spleens were collected in ice-cold PBS (5% FBS) and mechanically dissociated. The cells were strained (70 μm), RBC lysed (Life Technologies, A1049201; 2 min), and counted using a haemocytometer. Total CD8+ T cells were magnet sorted (Stem Cell, 19853A) and cultured (DMEM + FBS 10%, penicillin–streptomycin 1% + nonessential amino acids (Corning, 25-025-Cl) + vitamin + β-mercaptoethanol (Gibco, 21985-023) + l -glutamine (VWR, 02-0131) + sodium pyruvate (Corning, 25-000-Cl)). Cell purity was systematically confirmed after magnet sorting and the numbers of CD8+CD62Lhi were immunophenotyped by flow cytometry.
To generate cytotoxic T lymphocytes, 2 × 105 CD8+ T cells were seeded and stimulated for 48 h under Tc1 inflammatory conditions (2 μg ml−1 plate bounded anti-CD3 and anti-CD28 (Bio X Cell, BE00011, BE00151) + 10 ng ml−1 rIL-12 (BioLegend, 577008) + 10 μg ml−1 of anti-IL-4 (Bio X Cell, BE0045).
To generate cytotoxic T lymphocytes, 2 × 105 CD8+ T cells were seeded and stimulated for 48 h under Tc1 inflammatory conditions (2 μg ml−1 plate bounded anti-CD3 and anti-CD28 (Bio X Cell, BE00011, BE00151) + 10 ng ml−1 rIL-12 (BioLegend, 577008) + 10 μg ml−1 of anti-IL-4 (Bio X Cell, BE0045).
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