Gene specific taqman primer probe mixes

E. coli 91–100696 serotype K1 was a clinical isolate obtained from a patient with bacteremia due to biliary sepsis. LPS from E. coli serotype 0111:B4 was purchased from List Biological Laboratories (Campbell, CA). Ultrapure LPS-EB from E. coli serotype 0111:B4 was purchased from Invivogen (San Diego, CA). Colloidal carbon was prepared as previously described (Frevert et al., 2000 (link)). The polyclonal rabbit anti-versican antibody (β-GAG) was from Chemicon International (Temecula, CA). Gene-specific TaqMan primer-probe mixes used for quantitative real time PCR of versican, decorin, biglycan, Has1, Has2, Has3, hyaluronidase 1 and 2, and 18S mRNA were purchased from Applied Biosystems (Foster City, CA). IL-4, IL-13, and IL-10were purchased from Peprotech (Rocky Hill, NJ).

E. coli 91-100696 serotype K1 was a clinical isolate obtained from a patient with bacteremia due to biliary sepsis. LPS from E. coli serotype 0111:B4 was purchased from List Biological Laboratories (Campbell, CA). Ultrapure LPS-EB from E. coli serotype 0111:B4 was purchased from Invivogen (San Diego, CA). Colloidal carbon was prepared as previously described (Frevert et al. 2000 (link)). The polyclonal rabbit anti-versican antibody (β-GAG) was from Chemicon International (Temecula, CA). Gene-specific TaqMan primer-probe mixes used for quantitative real time PCR of versican, decorin, biglycan, Has1, Has2, Has3, hyaluronidase 1 and 2, and 18S mRNA were purchased from Applied Biosystems (Foster City, CA). IL-4, IL-13, and IL-10 were purchased from Peprotech (Rocky Hill, NJ).