Milli q ultrapure water meter

Bovine serum albumin (BSA), tetramethylbenzidine, and horseradish peroxidase were obtained from Merck (Merck, Darmstadt, Germany). Goat anti-mouse IgG was purchased from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). Nicarbazin was obtained from Biovet JSC (Peshtera, Bulgaria). Chloramphenicol, trimethoprim, and sulphanilamide were purchased from J&K Scientific Ltd. (Beijing, China). Coating antigen and monoclonal antibody 14D2 were prepared and provided by the Xiya Zhang research team at Henan Agricultural University. Trisodium citrate, aurichlorohydric acid, K₂CO₃, methanol, acetonitrile, and other chemical reagents were provided by Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China). The chicken sample was purchased from Darunfa Market (Hefei, China) and was verified to be negative by liquid chromatography–mass spectrometry.
Nitrocellulose membrane (Millipore 135) was obtained from Millipore AG (Millipore AG, Zug, Switzerland). A 96-well microplate was purchased from Yunpeng Technology Development Co., Ltd. (Xiamen, China). Blood filtration membrane, polyvinyl chloride plates, and absorbent paper were purchased from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). A Milli-Q Ultrapure water meter was obtained from Millipore (Massachusetts, USA). An HQ-6-Ⅱ vortex mixer was bought from Beijing North Tongzheng Biotechnology Development Co., Ltd. (Beijing, China).

A 2695 high-performance liquid chromatograph was equipped with an automatic sampler and a 2475 fluorescence detector (Waters Corp., US). An XW-80A vortex mixer (Shanghai Medical University Instrument Factory), a Milli-Q ultrapure water meter (Millipore Corp., US), and a Sorvall Biofuge Primo centrifuge (Thermo Fisher Scientific Corp., US) were used.
1-Benzylimidazole (99%) was purchased from Fluka Corp. (US). γ-Chloropropyl-trimethylsilane, naphthalene (99%, Nap), acenaphthene (98%, Acp), fluorene (98%, Flu), anthracene (98.5%, Ant), fluoranthene (98%, FL), and pyrene (98%, Pyr) were purchased from AccuStandard Corp. (US). Acetonitrile was purchased from Shanghai ANPEL Corp. (China). Other solvents and reagents were of analytical grade (SCRC, China). The water used in this experiment was redistilled. With a brown glass bottle, water samples were collected from seawater in Shazikou, Qingdao, Shandong Province, and preserved under 4°C. Concentrations of six kinds of standard PAH stock solutions were separately 1.0 mg·mL⁻¹, which were dissolved in acetonitrile, and the mixed standard stock solution was diluted to 10.0 µg·mL⁻¹ by acetonitrile. Subsequently, the solutions were stored at −20°C and diluted into standard working solutions of different concentrations by acetonitrile as needed.