Renal function was assessed by measuring sCr and CysC. Serial blood samples were obtained at 0, 6, 12, 24, 48, and 72 hours from presentation to the ED. After centrifugation at 3,600 rpm for 15 minutes, serum samples were stored at −20°C until used. CysC was measured by particle enhanced immunonephelometric assay (N Latex Cystatin C, Siemens, Marburg, Germany), and its measuring range was 0.76 mg/L to 1.44 mg/L. The results of CysC were blinded to the medical staffs during the study and did not affect the management of patients. sCr was measured by enzymatic assay (Vitros Crea; Ortho Clinical Diagnostics, High Wycombe, UK), and its normal range was 0.8 to 1.5 mg/dL. The biomarker assays were performed following manufacturer's instructions. As recommended by Kidney Disease Outcome Quality Initiative (KDOQI) guidelines, eGFR was calculated using the modification of diet in renal disease (MDRD) formula [30 (link)–34 (link)].
Vitros crea
Manufactured by Ortho Clinical Diagnostics
Sourced in United Kingdom, United States
The Vitros Crea is a laboratory diagnostic instrument designed for the measurement of creatinine levels in biological samples. It utilizes a dry slide technology to perform the analysis quickly and efficiently.
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Lab products found in correlation
2 protocols using vitros crea
1
Renal Function Assessment via Biomarkers
2
Urine Output Monitoring in ICU Patients
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All patients were screened upon ICU admission on all days of the week. In those meeting the inclusion criteria, and none of the exclusion criteria, the total amount of urine excreted between 0300 and 1100 hours every day was collected in a two litre urine container via the urinary catheter and sent to the local hospital pathology laboratory. The volume of urine and exact duration of urine collection were recorded. The creatinine concentration in the urine sample was assayed using the VITROS® CREA (Ortho-Clinical Diagnostics Inc., Rochester, NY, USA) slide method with the Vitros fusion 5.1 analyser.
Demographic and clinical data collected included age, ethnicity, gender, height, weight, diabetes status, baseline serum creatinine concentration (SCr), baseline glomerular filtration rate according to the CKD-EPI equation (eGFR CKD-EPI ), inotrope usage, Acute Physiology and Chronic Health Evaluation (APACHE) II score, Sequential Organ Failure Assessment (SOFA) score, length of ICU and hospital stay and admission diagnosis.
Demographic and clinical data collected included age, ethnicity, gender, height, weight, diabetes status, baseline serum creatinine concentration (SCr), baseline glomerular filtration rate according to the CKD-EPI equation (eGFR CKD-EPI ), inotrope usage, Acute Physiology and Chronic Health Evaluation (APACHE) II score, Sequential Organ Failure Assessment (SOFA) score, length of ICU and hospital stay and admission diagnosis.
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