Bd1047 dihydrobromide

Methamphetamine hydrochloride was purchased from Sigma-Aldrich (St. Louis, MO). M100907 was synthesized by Kenner Rice (Ullrich and Rice, 2000 (link)). BD 1047 dihydrobromide was purchased from Sigma-Aldrich. M100907 was dissolved in sterile water with dropwise addition of 0.1 N hydrochloric acid and sonication until it dissolved. All other drugs were dissolved in physiological saline. All drugs were administered by intraperitoneal (IP) injection at a volume of 0.01 ml per g body weight of each mouse. All doses are reported in the salt form 30 minutes prior to treatment with METH at 78 mg/kg (LD₆₇), mice were administered M100907 (1 and10 mg/kg), BD 1047 (10 mg/kg), or a combination of M100907 (1 mg/kg) and BD 1047 (10 mg/kg), which we subsequently refer to as M100907/BD 1047 (1/10 mg/kg).

Cocaine hydrochloride and BD1047 dihydrobromide (a highly selective putative Sig1R antagonist) were purchased from Sigma Aldrich (St. Louis, MO.), PRE-084 (2-(4-Morpholinethyl)-1 phenylcyclohexanecarboxylate hydrochloride) at 99.9% purity was obtained from Tocris Pharmaceuticals (Avonmouth, Bristol, UK). The cocaine dose selected for this study (10μM) was based on our previous studies and other viability assays (Zenón-Meléndez et al., 2014 (link)).