Pe tr

Manufactured by Thermo Fisher Scientific

The PE-TR is a laboratory instrument designed for automated DNA/RNA extraction and purification. It utilizes magnetic bead technology to isolate and purify nucleic acids from a variety of sample types. The core function of the PE-TR is to provide a streamlined and efficient process for extracting and purifying genetic material for further analysis and research applications.

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Lab products found in correlation

Fix perm kit, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Live dead fixable aqua, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD14-PE-TR (2 citations) CD8-PE-TR (2 citations) CD11b PE-TR (2 citations)

2 protocols using pe tr

Intracellular Cytokine Analysis in T Cells

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For intracellular cytokine analysis, re-stimulated cells were harvested and stained for surface markers and viability using antibodies directed against CD3 (V500; BD Pharmingen: 560770; clone SP34-2), CD8 (BV421; BD Horizon: 562428; clone RPA-T8), CD4 (PE-Cy7; BD Pharmingen: 557852; clone SK3/Leu3a), CD14 (PE-TR; Invitrogen: MHCD1417; clone TuK4), CD25 (APC; Miltenyi Biotec: 130-098-213; clone 4E3) and yellow fixable live-dead dye (Invitrogen). After surface staining, cells were permeabilized and fixed using the BD fix/perm kit and incubated with anti-IFN-γ (PE; BD Pharmingen: 554701; clone B27) labeled antibodies for 30 minutes at 4°C. In some experiments cells were stained for intracellular TNF-α (APC; BD Pharmingen: 340534; clone 6401.1111) and not surface CD25. Cells were washed and analyzed on the Aria II flow cytometer. When feasible, 30,000 events were taken up for each tube. Analyses were performed using FlowJo software version 9.7.6 (Treestar).

van de Ven R., Hilton T.L., Hu H.M., Dubay C.J., Haley D., Paustian C., Puri S., Urba W.J., Curti B.D., Aung S, & Fox B.A. (2018). Autophagosome-based strategy to monitor apparent tumor-specific CD8 T cells in patients with prostate cancer. Oncoimmunology, 7(12), e1466766.

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Immunophenotyping of NK and T Cells

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The following monoclonal antibodies (mAbs) were used (clone names are
given within parentheses): anti-CD3 (S4.1/7D6) PE-TR, anti-CD19 (SJ25-C1) PE-TR
(Invitrogen), anti-CD14 (MoP9) APC-Cy7, anti-CD16 (3G8) PacBlue, anti-CD56
(NCAM16) APC R700 (BD Pharmingen), anti-CD314 or NKG2D (1D11) (BioLegend), and
anti-CD159a or NKG2A (Z199) (Beckman Coulter). For phenotypic analysis, frozen
PBMCs were thawed, washed, and stained with mAbs at 4°C for 30 min.
LIVE/DEAD Fixable Aqua (Invitrogen) was used to determine viability. Samples
were acquired on a FACSCanto II or an LSR Fortessa (both BD Biosciences). Gating
strategies for NK cells and T cells are shown in Supplemental Figure 1 A-B. All flow cytometry
analyses were performed using FlowJo version 9 software (Tree Star, Inc.).

Zaffiri L., Messinger J.E., Bush E.J., Staats J.S., Patel P., Palmer S.M., Weinhold K.J., Snyder L.D, & Luftig M.A. (2023). Evaluation of host cellular responses to Epstein-Barr virus (EBV) in adult lung transplant patients with EBV-associated diseases. Journal of medical virology, 95(4), e28724.

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