Quercetin and other chemicals were purchased from Sigma (St. Louis, MO, USA) unless specified otherwise. Erlotinib (Tarceva, OSI Pharmaceuticals, Melville, NY, USA) was purchased from Lumtec (Hsinchu, Taiwan), antibodies against Akt, E-cadherin, GAPDH, p-Akt, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and anti-p-EGFR antibodies were purchased from Millipore (Burlington, MA, USA). Anti-EGFR, ERK, HK2, LDHA, MMP-2, PKM2, p27, Twist, and Vimentin antibodies were purchased from GeneTex, Inc. (Irvine, CA, USA). Antibodies against GLUT1, p-ERK, N-cadherin, and α-tubulin were obtained from Cell Signaling Technology (Danvers, MA, USA). MMP-9 and p21 antibodies were obtained from Abcan (Cambridge, UK) and Proteintech (Rosemont, IL, USA), respectively. PKM2 siRNA and the Lipofectamine RNAiMAX reagent were purchased from Invitrogen (Grand Island, NY, USA).
Pkm2 sirna
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PKM2 siRNA is a laboratory reagent designed to target and silence the expression of the PKM2 gene. It is a small interfering RNA (siRNA) molecule that can be used in experimental settings to study the role and function of the PKM2 protein.
Automatically generated - may contain errors
Lab products found in correlation
α tubulin, by Cell Signaling Technology (1 mentions)
Quercetin, by Merck Group (1 mentions)
P akt, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Anti egfr, by GeneTex (1 mentions)
E cadherin, by Santa Cruz Biotechnology (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Twist, by GeneTex (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Vimentin, by GeneTex (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 plasmid, by Thermo Fisher Scientific (1 mentions)
2 protocols using pkm2 sirna
1
Erlotinib and Quercetin Pathway Analysis
2
Regulation of Pyruvate Kinase M2 Expression
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For enforced expression of PKM2, PKM2 expression vector was conducted by cloning the open reading frame of the PKM2 gene into the pcDNA3.1 plasmid (Invitrogen, Carlsbad, CA, USA). For direct knockdown of PKM2, PKM2 siRNA was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). To perform the gain- and loss-of-function experiments of PKM2, we transient transfected cells with 2 μg/mL PKM2 plasmid or 50 pmol/mL PKM2 siRNA using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. Negative control oligonucleotides (NCOs), used as the general negative control RNA, were purchased from Genechem (Shanghai, China), and empty pcDNA3.1 plasmids were used as the internal control for transfection with PKM2 siRNA and PKM2 plasmids, respectively.
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