Tissues were fixed in 4% PFA overnight, washed with PBS, dehydrated in a gradient series of alcohol solutions, and embedded in paraffin. These paraffin-embedded tissues were then sectioned (5 μm) before being deparaffinized and rehydrated. Tissue sections were stained with hematoxylin, incubated in bluing solution, counterstained with eosin, dehydrated, and equilibrated with xylene. Glass coverslips were mounted with Permount Mounting Media (SP15-100; Thermo Fisher Scientific). Sections were photographed under bright-field microscope photograph system (Leica Microsystems).
Permount mounting media sp15 100
Manufactured by Thermo Fisher Scientific
Permount Mounting Media (SP15-100) is a liquid mounting medium used for the preparation of permanent microscope slides. It is designed to provide a transparent, durable, and refractive index-matched medium for the mounting of specimens.
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Lab products found in correlation
2 protocols using permount mounting media sp15 100
1
Paraffin-Embedded Tissue Staining
2
Hematoxylin and Eosin Staining Protocol
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Hematoxylin and eosin staining was performed as previously described (Li et al. 2014 (link)). Briefly, sections were dewaxed, rehydrated, stained with hematoxylin, incubated in bluing solution, counterstained with eosin, dehydrated, and equilibrated with xylene. Glass coverslips were mounted with Permount Mounting Media (SP15-100; ThermoFisher Scientific). Sections were photographed using a bright-field microscope system (Leica Microsystems).
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