Ferrous sulfate

The Rhodotorula glutinis SRY strain was isolated and maintained by the Food Microbiology Team, Agro-Processing Institute, Jilin Academy of Agricultural Sciences. Protein, yeast extract powder, agar powder, sodium chloride, glucose, concentrated hydrochloric acid, acetone, zinc sulfate, manganese sulfate, ferrous sulfate, copper sulfate, hydrogen peroxide and sodium bicarbonate were all purchased from Sangon Biotech (Shanghai) Co., Ltd.; vitamin B1 was purchased from Cisen Pharmaceutical Co., Ltd.; soybean oil was purchased from Yihai Kerry Arawana Holdings Co, Ltd. and TWW is taken from the local market.

The hFOB1.19 osteoblast cell line and KGN ovarian granulosa cell line were purchased from the Shanghai Institute of Cell Research, Chinese Academy of Sciences, and cultured at 37 °C with 5% CO₂. KGN cells were cultured in DMEM + 10% FBS + 1% double-antibody, while hFOB1.19 cells were cultured in DMEM + 10% FBS + 1% double-antibody. The cultured cells were used for follow-up treatment. The cells were treated with different concentrations of Rutin (0.01 mmol/L, 0.05 mmol/L and 0.1 mmol/L) (Selleck, Houston, TX, USA) for 48 h, followed by 100um ferrous sulfate (Sangon, Shanghai, China) and 600 um hydrogen peroxide (Sangon, China) for one hour. The cells were then collected for follow-up experiments.