Anti sodium potassium atpase

Manufactured by Abcam

Anti-sodium potassium ATPase is a laboratory equipment used to detect and quantify the presence of sodium-potassium ATPase, an enzyme responsible for maintaining the electrochemical gradient across the cell membrane. It is a crucial tool for researchers studying ion transport, cell signaling, and related biological processes.

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Lab products found in correlation

Protein a g magnetic beads, by Selleck Chemicals (1 mentions) Complete protease inhibitor cocktail tablet, by Roche (1 mentions) Anti glua1, by Abcam (1 mentions) Anti nlrp3, by Abcam (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Anti vcp p97, by Abcam (1 mentions) Anti glua2, by Abcam (1 mentions) Criterion tgx protein gel, by Bio-Rad (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions) Ecl primer western blotting detection reagent, by GE Healthcare (1 mentions) Anti β actin, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Anti inos antibody, by Abcam (1 mentions) Anti gapdh, by Merck Group (1 mentions) Snap i d system, by Merck Group (1 mentions) Trans blot semi dry transfer cell, by Bio-Rad (1 mentions) Sodium dodecyl sulphate polyacrylamide gels, by Bio-Rad (1 mentions) Anti lamin b1, by Abcam (1 mentions)

Spelling variants of this product

Anti-Sodium Potassium ATPase antibody (4 citations) Sodium potassium ATPase (2 citations) Anti-Alpha 1 sodium potassium ATPase (2 citations)

3 protocols using anti sodium potassium atpase

Isolation and Fractionation of Plasma Membrane Proteins

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Anti-NLRP3 (#ab91413), anti-caspase-1 (#ab1872), anti-IL-1β(#ab9722), anti-GluA1 (#ab31232), anti-GluA2 (#ab133477), anti-sodium potassium ATPase (#ab76020) and anti-VCP/p97 (#ab11433) antibodies were purchased from Abcam. Anti-β-actin antibody (#A5441) was obtained from Sigma-Aldrich. Anti-PSD-95 antibody (#MAB1598) was obtained from Millipore.
The Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (#SM-005) was purchased from Invent Biotechnologies, Inc. Roche Applied Science provided Complete Protease Inhibitor Cocktail Tablets (#04693116001). The Pierce™ BCA Protein Assay Kit (#23225) was purchased from Thermo Scientific. Protein A/G Magnetic Beads (#B23202) was purchased from Bimake. AC-YVAD-CMK peptide (#SML0429) was purchased from Sigma-Aldrich. For the in vitro experiments, cultured primary neurons were pretreated with AC-YVAD-CMK (5 µM) 1 h before oxygen-glucose deprivation (OGD). For the in vivo experiments, AC-YVAD-CMK (1 mg/kg, i.p.) was administered 1 h before HIBD surgery and then daily for 7 days.

Chen Y., Li X., Xiong Q., Du Y., Luo M., Yi L., Pang Y., Shi X., Wang Y.T, & Dong Z. (2023). Inhibiting NLRP3 inflammasome signaling pathway promotes neurological recovery following hypoxic-ischemic brain damage by increasing p97-mediated surface GluA1-containing AMPA receptors. Journal of Translational Medicine, 21, 567.

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CFTR Protein Expression Quantification

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Protein lysates were collected 48 hours after transient transfection of EMG or cDNA plasmids into HEK293 cells. 40μg of lysate were loaded per sample into a 7.5% Criterion TGX protein gel (BioRad). Transfer to PVDF membrane was performed in a Trans-Blot Turbo Transfer System (BioRad). After blocking, the membrane was probed with either mouse monoclonal anti-CFTR antibody 596 binding amino acids 1204–1211 (CFFT, University of North Carolina Chapel Hill) or mouse monoclonal anti-CFTR antibody 570 binding amino acids 731–742 (CFFT, University of North Carolina Chapel Hill) diluted to 1:5,000. Rabbit monoclonal anti-sodium/potassium-ATPase (Abcam) diluted 1:50,000 was used as a loading control. Secondary antibodies were anti-mouse (1:150,000 GE Healthcare) and anti-rabbit (1:100,000 GE Healthcare), respectively. Blots were exposed on film using ECL Primer Western Blotting Detection Reagent (GE Healthcare).

Joynt A.T., Evans T.A., Pellicore M.J., Davis-Marcisak E.F., Aksit M.A., Eastman A.C., Patel S.U., Paul K.C., Osorio D.L., Bowling A.D., Cotton C.U., Raraigh K.S., West N.E., Merlo C.A., Cutting G.R, & Sharma N. (2020). Evaluation of both exonic and intronic variants for effects on RNA splicing allows for accurate assessment of the effectiveness of precision therapies. PLoS Genetics, 16(10), e1009100.

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Western Blot Analysis of Cellular Proteins

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Samples containing 20 µg of cytosolic protein or 10 µg of nuclear and membranous protein were loaded in 4-20 % sodium dodecyl sulphate polyacrylamide gels (Biorad, Hercules, CA, USA). Proteins were transferred to PVDF membranes (Merck) with a Trans-Blot® semi-dry transfer cell (Biorad). The Snap i.d. system (Merck) was used for membrane blocking and antibody incubation as previously described 19 . Anti-Nrf2 primary antibody (1:1000, Merck), anti-NFkB-p65 (1:1000, Merck), anti-iNOS antibody (1:5000, Abcam, Cambridge, UK) and anti-gp91-phox (1:1000, Merck) were used to detect their corresponding proteins. Protein band intensity was corrected using anti-lamin B1 (1:5000, Abcam), anti-sodium potassium ATPase (1:10000, Abcam), anti-β-actin (1:10000, Merck) and anti-GAPDH (1:5000, Merck) in nuclear, membranous, and cytosolic fractions, respectively. The immunoreactive bands were detected with Supersignal West Pico Luminiscent Substrate and Supersignal West Femto Maximum Sensitivity Substrate. Diversity GeneSnap system and software (Syngene, Cambridge, U.K.) were used for protein bands detection. Experiments were performed at least three independent times by duplicate.

Alvariño R., Alonso E., Tabudravu J.N., Pérez-Fuentes N., Alfonso A, & Botana L.M. (2021). Tavarua Deoxyriboside A and Jasplakinolide as Potential Neuroprotective Agents: Effects on Cellular Models of Oxidative Stress and Neuroinflammation. ACS chemical neuroscience, 12(1).

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