The microcapsule suspension was filtered, washed for times using DI water, and dried for 12 h in a vacuum oven at 50 °C to obtain a microcapsule sample without any non-encapsulated essence oil and free water. All the samples were analyzed using a Thermo Nicolet 380 FTIR spectrometer (Thermo Fisher Scientific) using the KBr pellet method (transmittance mode, range = 400–4000 cm−1, resolution 4 cm−1).
Thermo nicolet 380 ftir spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Thermo Nicolet 380 FTIR spectrometer is a laboratory instrument designed for infrared spectroscopy analysis. It utilizes Fourier transform infrared (FTIR) technology to measure the absorption and emission characteristics of various samples. The core function of the Thermo Nicolet 380 is to provide high-resolution infrared spectra for the identification and characterization of materials.
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4 protocols using thermo nicolet 380 ftir spectrometer
1
FTIR Analysis of Microcapsules
2
FTIR Analysis of KLE Functional Groups
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FTIR was performed using a Thermo Nicolet 380 FTIR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) to identify the functional group of the KLE. The sample preparation for the study was performed in the same way as described in Section 3.4.1 . The extract and the coating samples were analyzed on a KBr disk under infrared ray beams ranging from 4000 to 400 cm−1 with the spectrum resolution of 4 cm−1. The FTIR data were recorded in the transmittance mode. The transmittance of the infrared rays at different frequencies was translated into an IR absorption plot. The spectral pattern was analyzed and matched according to the IR absorption table to identify the functional groups of the constituents in the extract and the coatings prepared.
3
Characterization of Modified Carbon Nanowhiskers
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Infrared spectroscopy was used to determine functional group absorption bands in the modified CNWs on a Thermo-Nicolet 380 FTIR spectrometer (Thermo Fisher Scientific Inc., USA) in transmission mode. Two mg of the solid samples (CNWs) were milled with 200 mg potassium bromide (KBr) to form a very fine powder using agate pestle and mortar. This powder was then compressed to form a thin transparent disk at 10 metric tonnes pressure for FTIR analysis. Zeta potential of particles was measured with a Malvern Instrument Nano-ZS Zetasizer (Malvern Instrument Ltd., Worcestershire, UK). Five mg of the solid samples (CNWs) was diluted in 5 mL of deionised water and sonicated for 10 min before analysis.
4
Determining Chitosan Deacetylation Degree
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Spectroscopy FTIR spectra of chitosan, were recorded using a Fourier transform infrared spectrophotometer (Thermo Nicolet 380 FT-IR spectrometer, Thermo Fisher Scientific, Loughborough, UK), operating from 4000 to 400 cm -1 . The degree of deacetylation (DD) was calculated from equation 7 [35] .
(%) = 100 -( . ) (Eq. 7)
where A1655 cm -1 and A3450 cm -1 are the areas under the absorbance peaks for the C=O and OH stretches, respectively.
(%) = 100 -( . ) (Eq. 7)
where A1655 cm -1 and A3450 cm -1 are the areas under the absorbance peaks for the C=O and OH stretches, respectively.
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