4000 gc ms mass spectrometer

The chemical composition of the extracted VOO was investigated by the solid-phase microextraction–gas chromatography–mass spectrometry (SPME/GC/MS) technique using a Varian 4000 GC/MS mass spectrometer [27 (link)]. A capillary column VF 5ms (60 m × 0.25 mm ID, 0.25 μm film thickness) was used to fractionate the oil samples. Operating conditions were as follows: split ratio, 50:1; He gas flow 1.5 mL/min; injection volume 1.0 μL; column temperature maintained at 40 °C for 10 min, then raised to 180 °C at 20 °C/min, then raised to 220 °C at 10 °C/min; injector, transfer line, and ion source at temperatures were 250 °C, 270 °C, and 200 °C, respectively; pre-incubation time 20 min at 40 °C, and desorption time 5 min. The mean of the data was calculated from three biological repeats obtained from three independent experiments. Electron impact mass spectra (EI-MS) were recorded at ionization energy of 70 eV, 2 scan/s. The VOO’s chemical ingredients were identified by matching the mass spectral patterns and retention times of these ingredients to those of standard compounds or by comparing their mass spectra to those in the Wiley 6th edition mass spectra collection. In addition, the Kováts indices were calculated and compared to published retention indices. Compounds were quantified based on their area in the chromatogram.

Solid phase microextraction–gas chromatography–mass spectrometry (SPME/GC/MS) was used to analyze the extracted VOO for its chemical composition with a Varian 4000 GC/MS mass spectrometer [35 (link)]. The oil samples were fractionated using a VF 5ms capillary column (60 μm × 0.25 mm ID, 0.25 μm film thickness). The working conditions were as follows: He gas flow rate of 1.5 mL/min; injection volume of 1.0 L; split ratio of 50:1. The column was kept at 40 degrees Celsius for 10 min, then heated to 180 degrees Celsius at a rate of 20 °C/min, and finally heated to 220 degrees Celsius at a rate of 10 °C/min; temperatures of 250 °C, 270 °C, and 200 °C were used for the injector, transfer line, and ion source, respectively; twenty minutes at 40 °C for pre-incubation; five minutes for desorption. Data were averaged from three biological replicates from three separate studies. At an ionization energy of 70 eV, 2 scans/s, electron impact mass spectra (EI-MS) were acquired. The chemical components of the VOO were specified by comparing their respective retention times and MS patterns to those of standard compounds recorded in the Wiley MS collection 6th edition. VOO compounds were quantified according to the area of their respective peaks in the GC chromatogram.