Immunohistochemical stainings were performed as described previously [52] (link), using the monoclonal primary anti-NUT antibody (clone C52B1, 1:200, Cell Signaling Technology, Danvers, MA, USA) and the monoclonal anti-NR4A3 (NOR-1) antibody (clone H-7, 1:25, Santa Cruz Biotechnology, Inc, Dallas, TX, USA), applying the automated Leica Bond III staining system (Leica Biosystems, Nussloch, Germany). The Ventana Benchmark staining system (Oro Valley, AZ, USA) was applied for the following primary antibodies: p63 (clone 4A4, prediluted, Ventana, Oro Valley, AZ, USA), p40 (clone BC24, 1:100, Zytomed Systems, Berlin, Germany), panTRK (clone EPR17341, 1:100, Abcam, Cambridge, UK), CD117 (clone YR145, 1:200, Cell Marque Lifescreen Ltd., Rocklin, CA, USA), Ki-67 (clone 30-9, prediluted, Ventana, Oro Valley, AZ, USA), S100 (polyclonal, 1:2000, DAKO A/S, Jena, Germany), DOG-1 (clone SP31, 1:50 Invitrogen, Carlsbad, CA, USA).
P63 clone 4a4
Manufactured by Roche
Sourced in United States
The P63 (clone 4A4) is a laboratory reagent used in immunohistochemistry applications. It is a mouse monoclonal antibody that specifically binds to the p63 protein, which is a member of the p53 family of transcription factors. The P63 (clone 4A4) can be used to detect the presence and localization of p63 in various cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Ventana benchmark xt, by Roche (2 mentions)
Bond 3 staining system, by Leica (1 mentions)
Ki 67 clone 30 9, by Roche (1 mentions)
Pantrk clone epr17341, by Abcam (1 mentions)
Herceptest kit, by Agilent Technologies (1 mentions)
Ck20 clone ks20, by Agilent Technologies (1 mentions)
P53 clone do 7, by Agilent Technologies (1 mentions)
Clone ov tl 12 30, by Agilent Technologies (1 mentions)
Ck7 clone ov tl 12 30, by Agilent Technologies (1 mentions)
Ttf 1 8g7g3 1, by Agilent Technologies (1 mentions)
3 protocols using p63 clone 4a4
1
Immunohistochemical Staining Protocols
2
Immunohistochemical Analysis of HER2 and Other Markers
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Immunohistochemical staining for HER2 was performed using the HercepTest™ kit (Dako, Glostrup, Denmark) according to manufacturer's protocols. 4 μm thick sections taken both from original full tumor blocks and the TMA block were used for HER2 IHC. HER2 protein expression was scored as 0, 1+, 2+, and 3+ according to the ASCO/CAP 2013 HER2 test guideline [26 (link)]. HER2 IHC 3+ was considered to be HER2-positive, IHC 2+ HER2-equivocal, and IHC 0 and 1+ HER2-negative [26 (link)]. Additional immunohistochemical staining for cytokeratin (CK) 7 (clone OV-TL 12/30; Dako), CK20 (clone Ks20.8; Dako), p53 (clone DO7; Dako), p63 (clone 4A4; Ventana), and CD138 (clone 5F7; Novocastra) was also performed on sections taken from the TMA block. All IHC were performed using Ventana Benchmark XT automated staining system (Ventana Medical Systems, Tucson, AZ).
3
Immunohistochemistry Staining Protocol for Tissue Analysis
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Immunohistochemical staining was performed on 4-μmthick sections, cut from formalin-fixed, paraffin-embedded tissue, using a Ventana BenchMark XT automated stainer (Ventana Medical Systems, Tucson, AZ), according to the manufacturer'sp r o t o c o l .
The following antibodies were used: S-100 protein (polyclonal; RTU; Ventana), STAT5 (E289, 1:500, AbCam), mammaglobin (clone 304-1A5;R T U ;D a k o C y t o m a t i o n ) , NTRK (A7H6R, 1:25; Cell Signaling), GATA3 (clone L50-823; 1:200; BioCareMedical), CK7 (clone OV-TL 12/30; 1:200; DakoCytomation), TTF-1 (8G7G3/1, 1:100, Dako), p63 (clone 4A4; RTU; Ventana), and CD117 (polyclonal, 1:800, Dako). The panel varied between individual cases depending on the origin.
The following antibodies were used: S-100 protein (polyclonal; RTU; Ventana), STAT5 (E289, 1:500, AbCam), mammaglobin (clone 304-1A5;R T U ;D a k o C y t o m a t i o n ) , NTRK (A7H6R, 1:25; Cell Signaling), GATA3 (clone L50-823; 1:200; BioCareMedical), CK7 (clone OV-TL 12/30; 1:200; DakoCytomation), TTF-1 (8G7G3/1, 1:100, Dako), p63 (clone 4A4; RTU; Ventana), and CD117 (polyclonal, 1:800, Dako). The panel varied between individual cases depending on the origin.
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