Mi se 7

Eight-week-old male BALB/c mice were randomized into three groups: 50 μl PBS group (n = 3/group), 50 μl PKH26 group, and 50 μl PKH26-labeled ASC-MVs group (c = 1.0 μg/μl). The wounds on mice back (1 × 1 cm) were subcutaneously injected with PBS, PKH26, or PKH26-labeled ASC-MVs once after the wounds were created. Fluorescence images were taken at days 1, 3, 5, 7, 10, and 15 by the in vivo imaging system (Bruker, German). The images were analyzed using Bruker MI SE 7.2 software.

Nine female SD rats aged 8 weeks were randomly divided into 3 groups (n = 3/group): group 1 was periurethrally injected with 100 μl PBS, group 2 was injected with 100 μl DiI, and group 3 was injected with 100 μl DiI-labeled sEV (1 μg/μl). Fluorescence images were taken on days 1, 2, 3, 5, 7, 10, and 14 using the in vivo imaging system (Bruker, German). The images were analyzed by the Bruker MI SE 7.2 software.

The nude BALB/c male mice (18–22 g) were obtained from the Laboratory Animal Center of Southern Medical University (Guangzhou, China). The animal protocols were approved by the Institutional Animal Care and Use Committee of Southern Medical University. A total of U87MG-EGFRvIII cells (1 × 10⁷) were subcutaneously injected into the right back flank of the nude mice for 10 days. The animals were divided randomly into five groups and SMUZ106 hydrochloride (75, 100, and 150 mg/kg/d), gefitinib (100 mg/kg/d), or saline solution were orally administered for 3 weeks. Tumor volumes were measured on every other day and the body weights of mice were measured daily.
Through an intraperitoneal injection of sodium pentobarbital, the nude mice were anesthetized and then immobilized. U87MG-EGFRvIII luciferase-transfected cells (5 × 10⁵ in 5 μL of PBS) were injected to a depth of 3 mm using a Hamilton syringe with a 26-gauge needle. After five days, the tumor growth rate was monitored using bioluminescence imaging in an In-Vivo MS FX PRO (Bruker, MA, USA), subsequent to an injection of luciferin (200 μL of 15 mg/mL) into the mice. Based on the tumor volume, the mice were randomized into two groups; the choice of vehicle or SMUZ106 hydrochloride (300 mg/kg/d) was administered by oral gavage. Additionally, the tumor volume was measured and calculated using Bruker MI SE 7.2 software.