Cryptochlorogenic acid

Manufactured by Chengdu Must Bio-Technology

Sourced in China

Cryptochlorogenic acid is a naturally occurring compound found in various plant species. It is a type of chlorogenic acid, a family of polyphenol compounds. Cryptochlorogenic acid serves as an antioxidant and has been studied for its potential biological activities, though its specific functions and applications require further research.

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Lab products found in correlation

4 protocols using cryptochlorogenic acid

Quantification of Phenolic Compounds in Semen Cuscutae

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Methanol and acetonitrile (HPLC pure grade) were purchased from Fisher Co., Ltd. Formic acid was of chromatographic purity obtained from ROE Co., Ltd. Ultrapure water for the HPLC-MS/MS analysis was purified by Milli-Q water purification system (Millipore, Milford, MA, USA). Hyperoside, caffeic acid, rutin, chlorogenic acid, luteolin, apigenin, kaempferol, isoquercitrin, cryptochlorogenic acid, isorhamnetin-3-O-glucoside, astragalin, and liquiritin (internal standards, IS) were obtained from Chengdu Must Bio-Technology Co., Ltd (Chengdu, China). Quercitrin and quercetin were purchased from National Institutes for Food and Drug Control. Semen Cuscutae was purchased from Anguo, Hebei province. The structures of 13 compounds are displayed in Figure 1.
Sprague–Dawley rats (SPF, 200 ± 10 g, male) were purchased from HFK Laboratory Animal Technology Co., Ltd (license number: SCXK 2014-0004).

Shen J., Jia Q., Huang X., Yao G., Ma W., Zhang H., Ouyang H, & He J. (2019). Development of a HPLC-MS/MS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 6518528.

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Angelica pubescens Maxim. f. biserrata Root Analysis

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HPLC grade methanol and acetonitrile were obtained from Fisher Scientific (USA). HPLC grade formic acid was purchased from Dikma Co. (USA), and Wahaha purified water was obtained from Hangzhou Wahaha Group (China). Neo chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B (3,4-diCQA), isochlorogenic acid A (3,5-diCQA), isochlorogenic acid C (4,5-diCQA), bergapten, columbianetin acetate, isoimperatorin, osthol, and columbianadin were obtained from Chengdu Must Biotechnology Co., LTD (China), and columbianetin, xanthotoxin, psoralen, and chloramphenicol were obtained from Shanghai Nature Standard Biotechnology Co., LTD (China), and daidzein was obtained from Chengdu Purechem-Standard Biotechnology Co., LTD (China). The purity of the involved standards and reagents were over 98%, with their chemical structures exhibited in Figure 1.
The APR samples was obtained from Hubei Province (30°24N, 115°30E) picked in April 2019, and identified by Professor Lianjie Su from Heilongjiang University of Chinese Medicine, as the dry root of Angelica pubescens Maxim. f. biserrata Shan et Yuan and a voucher specimen were deposited at department of Chinese herbal medicine processing of Heilongjiang University of Chinese Medicine, Harbin, China (voucher numbers: APQH201904-7).

Hou A., Yu H., Wang S., Zhang J., Wang X., Zheng S., Yang L., Kuang H, & Jiang H. (2022). Comparison of Pharmacokinetic Profiles of 14 Major Bioactive Components in Normal and Arthritic Model Rats after Oral Administration of Angelicae pubescentis Radix by UPLC-MS/MS. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 8379921.

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Protein-Ligand Interaction Assay

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BSA was purchased from AMRESCO. Chlorogenic acid, cryptoChlorogenic acid and neoChlorogenic acid were all purchased from Chengdu Must Bio-Technology Company in China. Ibuprofen and Warfarin Sodium were purchased from Shanghai Yuanye Bio-Technology Company in China. Digoxin was purchased from Tokyo Chemical Industry Company in Japan. BSA solution was prepared to 1.0 × 10⁻⁴ M with water. PBS and Tris-HCl buffer were prepared to 5.0 × 10⁻² M, all the solution were kept at pH 7.4 and contain 0.1 M NaCl. The stock solution of 3-CQA, 4-CQA, 5-CQA, ibuprofen, warfarin sodium, and digoxin were directly dissolved in MeOH as stock solution (1.0 × 10⁻³ M).

Jiao Q., Zhang W., Jiang Y., Jiang L., Chen X, & Liu B. (2019). Study on the Interactions Between Caffeoylquinic Acids With Bovine Serum Albumin: Spectroscopy, Antioxidant Activity, LC-MSn, and Molecular Docking Approach. Frontiers in Chemistry, 7, 840.

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Isolation and Purification of Bioactive Compounds

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Chlorogenic acid, cryptoChlorogenic acid, caffeic acid, isoChlorogenic acid A, isoChlorogenic acid B and isoChlorogenic acid C (purity > 98%) were purchased from Chengdu Must Bio-Technology Co., LTD (Chengdu, China) while rutin (purity > 98%) was purchased from National Institutes for Food and Drug Control of China (Beijing, China); kaempferol-3-Orutinoside and 3-methoxyquercetin (purity > 98%) were purchased from Shanghai Tanto Biotech Co., LTD (Shanghai, China) and brevilin A(purity > 90%) was purchased from BioBiopha Co., LTD (Kunming, China). Brevilin A was further purified by preparative HPLC and the final purity of brevilinA was 97.7% as determined by HPLC-DAD at 224 nm. Arnicolide D and arnicolide C were obtained by isolation using preparative HPLC and the chemical structures were confirmed by 1 H-NMR and 13 C-NMR spectral analysis. HPLC grade acetonitrile and acetic acid were purchased from Thermo Fisher Scientific Inc., Tedia (USA) and Acros Organics, respectively. Ethanol (Guangdong Chemical Reagent Engineering-technological Research and Development Center, Guangzhou, China) was of analytical-reagent grade. Double deionized water was purified by Milli-Q water system (Millipore Corp., Bedford, MA, USA) and used to prepare all buffer and sample solutions.

Chan C.O., Jin D.P., Dong N.P., Chen S.B, & Mok D.K. (2016). Qualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD. Journal of pharmaceutical and biomedical analysis, 125.

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