The total levels of Aβ40 and Aβ42 in the brain homogenates were determined using sandwich ELISA. Briefly, 150 mg of brain tissue was polytron homogenized in and extracted with 70% formic acid and centrifuged for 2 h at 15,000× g at 4 °C. The supernatant was collected and diluted 1:20 with TB buffer (1 M Tris base, 0.5 M NaHPO4) for neutralization, followed by further dilution 1:1 with antigen capture buffer provided by the ELISA kit. The total Aβ40 and Aβ42 were determined using anti-human Aβ40 and Aβ42 ELISA kits (Thermo Fisher Scientific, Carlsbad, CA, USA). All samples were run in duplicates for n = 7 mice/treatment. The total Aβ concentrations were expressed as the pg/g brain tissue weight.
Anti human aβ40 and aβ42 elisa kits
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-human Aβ40 and Aβ42 ELISA kits are designed for the quantitative determination of amyloid-beta (Aβ) 40 and Aβ42 levels in human biological samples. These kits utilize a sandwich enzyme-linked immunosorbent assay (ELISA) technique.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti human aβ40 and aβ42 elisa kits
1
Quantification of Brain Amyloid-β Levels
2
Quantifying Soluble Aβ40 and Aβ42 in 5XFAD Mice
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