Total proteins were collected from the cell samples after lysis with a lysis buffer (RIPA buffer, Solarbio, Beijing, China) supplemented with protease inhibitors (Solarbio, Beijing, China), phosphatase inhibitors (Solarbio, Beijing, China), and phenylmethylsulfonyl fluoride (Solarbio, Beijing, China). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was performed on a 10% gel. After filtration through a nitrocellulose membrane (Solarbio), the membrane was sealed with 5% skim milk and treated with primary antibodies (anti-HSD17B12, 1:500, Thermo Fisher Scientific, Shanghai, China; anti-β-actin, 1:3000, Immunoway, Beijing, China) and secondary antibodies. Finally, the membranes were imaged using an Odyssey Clx Imaging System (LICOR, Lincoln, NE, USA) and quantified using Image-Pro Plus software (Media Cybernetics, Rockville, MD, USA).
Anti hsd17b12
Manufactured by Thermo Fisher Scientific
Sourced in China
Anti-HSD17B12 is a laboratory reagent used for the detection and quantification of the HSD17B12 protein in biological samples. HSD17B12 is an enzyme involved in the biosynthesis of fatty acids and steroids. The Anti-HSD17B12 reagent can be used in various research applications that require the analysis of HSD17B12 expression or activity.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor, by Solarbio (2 mentions)
Nitrocellulose membrane, by Solarbio (2 mentions)
Phosphatase inhibitor, by Solarbio (2 mentions)
Odyssey clx imaging system, by LI COR (2 mentions)
Anti β actin, by Immunoway (2 mentions)
Image pro plus software, by Media Cybernetics (2 mentions)
Ripa buffer, by Solarbio (2 mentions)
Phenylmethylsulfonyl fluoride, by Solarbio (2 mentions)
2 protocols using anti hsd17b12
1
Western Blot Analysis of HSD17B12
2
Western Blot Analysis of HSD17B12
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total proteins were collected from the cell samples after lysis with a lysis buffer (RIPA buffer, Solarbio, Beijing, China) supplemented with protease inhibitors (Solarbio, Beijing, China), phosphatase inhibitors (Solarbio, Beijing, China), and phenylmethylsulfonyl fluoride (Solarbio, Beijing, China). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was performed on a 10% gel. After filtration through a nitrocellulose membrane (Solarbio), the membrane was sealed with 5% skim milk and treated with primary antibodies (anti-HSD17B12, 1:500, Thermo Fisher Scientific, Shanghai, China; anti-β-actin, 1:3000, Immunoway, Beijing, China) and secondary antibodies. Finally, the membranes were imaged using an Odyssey Clx Imaging System (LICOR, Lincoln, NE, USA) and quantified using Image-Pro Plus software (Media Cybernetics, Rockville, MD, USA).
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