Acetyl l carnitine alcar

For chronic treatment, we had six groups of male rats. The, first three groups received saline (n= 5), D-carnitine (DC, Sigma, St Louis, MO, USA) (n= 5) at dose of 500mg/kg/day, intraperitoneal (i.p.) for carnitine depletion as mentioned earlier [20] , or acetyl-L-carnitine (ALCAR, Sigma, St Louis, MO, USA) (n= 5) at a dose of 300mg/kg/day, i.p. for 15 days. In the last three groups, rats had pretreatment conditions for 5 days with saline, DC (500mg/kg/day, i.p.), or ALCAR (300mg/kg/day, i.p.). Then, they were treated with InG for 10 days with saline (InG+saline) (n= 6), DC (InG+DC) (n= 5) or ALCAR (InG+ALCAR) (n= 6), respectively. At the day of surgery, all rats were anesthetized with ketamine and xylazine (ketamine 100mg/kg and xylazine 10mg/kg, i.p.).
Then, rat's chest was opened by aseptic and sharp surgical scissors and forceps. Blood samples were collected directly from the heart. Rats were euthanized by exsanguination from the heart. Throughout the treatment course, BG levels, blood pressure and heart rate were monitored before (day 0) and during treatment (day 3, 6 and 9).