Adherent CHOK1 cell line (ATCC CRL-9661) was granted by Pasteur Institute of Iran. Ham’s F12 nutrient mixture was obtained from Inoclon Co. (Iran). Antibiotic solution of Penicillin/Streptomycin, and trypsin 10X were obtained from Biosera Co. (England). Fetal bovine serum was purchased from Gibco Co. (USA). High G content alginic acid (MW, 100 000-200 000, G content, 70%), calcium chloride, linear PEI (Mn~10 000), PLL (MW 70 000-150 000, 0.01%), HEPES, MTT reagent, trypan blue, fluorescein diacetate, propidium iodide, L-glutamine, and DMSO were purchased from Sigma-Aldrich (USA).
Antibiotic solution of penicillin streptomycin
Manufactured by Biosera
Sourced in United Kingdom
Antibiotic solution of Penicillin/Streptomycin is a commonly used sterile solution for cell culture applications. It contains a combination of the antibiotics penicillin and streptomycin to prevent bacterial contamination in cell culture media.
Automatically generated - may contain errors
Lab products found in correlation
Mtt reagent, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Hepes, by Merck Group (2 mentions)
Calcium chloride, by Merck Group (2 mentions)
Alginic acid, by Merck Group (2 mentions)
Trypsin 10x, by Biosera (2 mentions)
Fluorescein diacetate, by Merck Group (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Trypan blue, by Merck Group (1 mentions)
Linear pei, by Merck Group (1 mentions)
2 protocols using antibiotic solution of penicillin streptomycin
1
Adherent CHOK1 Cell Culture Protocol
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Adherent CHOK1 cell line (ATCC CRL-9661) was granted by Pasteur Institute of Iran. Ham’s F12 nutrient mixture was obtained from Inoclon Co. (Iran). Antibiotic solution of Penicillin/Streptomycin, and trypsin 10X were obtained from Biosera Co. (England). Fetal bovine serum was purchased from Gibco Co. (USA). High G content alginic acid (MW, 100 000-200 000, G content, 70%), calcium chloride, linear PEI (Mn~10 000), PLL (MW 70 000-150 000, 0.01%), HEPES, MTT reagent, trypan blue, fluorescein diacetate, propidium iodide, L-glutamine, and DMSO were purchased from Sigma-Aldrich (USA).
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Adherent CHOK1 cell line (ATCC CRL-9661) was granted by Pasteur Institute of Iran. Ham’s F12 nutrient mixture was obtained from Inoclon Co. (Iran). Antibiotic solution of Penicillin/Streptomycin, and trypsin 10X were obtained from Biosera Co. (England). Fetal bovine serum was purchased from Gibco Co. (USA). High G content alginic acid (MW, 100 000-200 000, G content, 70%), calcium chloride, linear PEI (Mn~10 000), PLL (MW 70 000-150 000, 0.01%), HEPES, MTT reagent, trypan blue, fluorescein diacetate, propidium iodide, L-glutamine, and DMSO were purchased from Sigma-Aldrich (USA).
2
Cytotoxicity Evaluation of Alginic Acid Hydrogels
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High G content alginic acid (MW, 100 000–200 000, G content, 70%), calcium chloride, branched and linear PEI (Mn ∼10 000), PLL (MW 70 000–150 000, 0.01%), HEPES, MTT reagent, and DMSO were purchased from Sigma-Aldrich (USA). Cell culture media (RPMI), antibiotic solution of penicillin/streptomycin, and trypsin 10X were obtained from Biosera Co. (England). Fetal bovine serum was purchased from Gibco Co. (USA). HepG2 cell line (NCBI Code: C158) was obtained from Pasteur Institute of Iran.
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